CXCL3/GRO Gamma Polyclonal antibody

• 

  WB analysis using 11221-1-AP

  Non-treated, LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 11221-1-AP (CXCL3/GRO Gamma antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CXCL3/GRO Gamma Polyclonal Antibody for WB, ELISA

Cat No. 11221-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Synonyms

C X C motif chemokine 3, CINC 2b, CXCL/GRO Gamma, CXCL3, GRO gamma, GRO gamma(1 73), GRO3, GROg, Growth regulated protein gamma, MIP 2b, MIP2 beta, MIP2B, SCYB3

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Tested Applications

• Positive WB detected in: LPS and Protein transport inhibitor treated THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

11221-1-AP targets CXCL3/GRO Gamma in WB, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CXCL3/GRO Gamma fusion protein Ag1732
• Full Name: chemokine (C-X-C motif) ligand 3
• Calculated Molecular Weight: 11 kDa
• Observed Molecular Weight: 8-10 kDa
• GenBank Accession Number: BC016308
• Gene Symbol: CXCL3
• Gene ID (NCBI): 2921
• RRID: AB_3669124
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P19876
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CXC chemokine ligand 3 (CXCL3) is a member of CXC-type chemokine family that is identified as a major regulator in immune and inflammation responses. CXCL3 is broadly expressed in various human tumor types, and it is also known to play a critical role in mediating tumor development and progression.

Publications

Species	Application	Title
human	IF	Nat Commun; Single-cell RNA sequencing highlights the role of inflammatory cancer-associated fibroblasts in bladder urothelial carcinoma.; Authors - Zhaohui Chen