Anti-Mouse CXCL2 Rabbit Recombinant Antibody

• 

  FC experiment of RAW 264.7 using 98259-1-RR

  1x10^6 untreated or LPS and Brefeldin A treated RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse CXCL2 Rabbit Recombinant Antibody (98259-1-RR, Clone: 242007G5) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 98259-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98259-1-RR against Mouse CXCL2 were performed. The affinity constant is below 1 pM.

CXCL2 {ptg:Brand} Recombinant Antibody for FC (Intra)

Cat No. 98259-1-RR

Clone No.242007G5

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Synonyms

Scyb2, Mip-2, MIP2, Macrophage inflammatory protein 2, C-X-C motif chemokine 2

---

Tested Applications

• Positive FC (Intra) detected in: LPS and Brefeldin A treated RAW 264.7 cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98259-1-RR targets CXCL2 in FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: chemokine (C-X-C motif) ligand 2
• Calculated Molecular Weight: 11 kDa
• GenBank Accession Number: NM_009140
• Gene Symbol: Cxcl2
• Gene ID (NCBI): 20310
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P10889
• Storage Buffer: PBS with 0.09% sodium azide , pH 7.3
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CXCL2 is a member of the CXC subfamily, which encodes secreted proteins involved in immunoregulatory and inflammatory processes. CXCL2 is expressed at sites of inflammation and may suppress hematopoietic progenitor cell proliferation. CXCL2 plays an critical role in maintaining cancer-induced macrophage infiltration and the resulting mechanical hypersensitivity and persistent spontaneous nociception (PMID: 36754246).