CXCL2 Polyclonal antibody

CXCL2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 26791-1-AP

Synonyms

C X C motif chemokine 2, CINC 2a, CXCL2, Gro beta, GRO beta T, GRO2, GROb, Growth regulated protein beta, HSF, MGSA b, MIP 2a, MIP2, MIP2 alpha, MIP2A, SB 251353, SCYB2

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Validation Data Gallery

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  WB analysis using 26791-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26791-1-AP (CXCL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26791-1-AP

  human milk was subjected to SDS PAGE followed by western blot with 26791-1-AP (CXCL2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HepG2 cells, human milk, rat lung tissue, mouse lung tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 3 publications below

Product Information

26791-1-AP targets CXCL2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CXCL2 fusion protein Ag25376
• Full Name: chemokine (C-X-C motif) ligand 2
• Calculated Molecular Weight: 107 aa, 11 kDa
• Observed Molecular Weight: 11 kDa
• GenBank Accession Number: BC015753
• Gene Symbol: CXCL2
• Gene ID (NCBI): 2920
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CXCL2, also known as macrophage inflammatory protein 2-alpha (MIP2-alpha), is a member of CXC chemokine family that binds to CXC chemokine receptor 2 (CXCR2). CXCL2 is mainly produced by macrophages, endothelial cells, epithelial cells and tumor cells. CXCL2 plays important roles in various biological progresses such as angiogenesis, inflammation, immune response and cancer biological behaviors. Under inflammatory conditions in CNS, microglia and astrocytes may secrete CXCL2 as well as other chemokines, which induces chemotaxis and infiltration of circulatory neutrophils. Indeed, previous studies suggest that CXCL2 is involved in Alzheimer disease, multiple sclerosis and brain ischemia.

Publications

Species	Application	Title
mouse	IF	Acta Pharmacol Sin; A novel resveratrol analog upregulates sirtuin 1 and inhibits inflammatory cell infiltration in acute pancreatitis.; Authors - Zheng-Nan Ren
mouse	IF	Int J Mol Sci; MyD88 in Macrophages Enhances Liver Fibrosis by Activation of NLRP3 Inflammasome in HSCs.; Authors - Shuang Ge
mouse	WB	J Immunol; Macrophagic Extracellular Vesicle CXCL2 Recruits and Activates the Neutrophil CXCR2/PKC/NOX4 Axis in Sepsis.; Authors - Guozhen Wang
mouse	WB	Int Immunopharmacol; Isoliquiritigenin alleviates LPS/ D-GalN-induced acute liver failure by activating the PGC-1α/ Nrf2 pathway to reduce oxidative stress and inflammatory response.; Authors - Lu Wang
mouse	IF	Hum Cell; Crosstalk among proximal tubular cells, macrophages, and fibroblasts in acute kidney injury: single-cell profiling from the perspective of ferroptosis; Authors - Yulin Wang