CXCL2 Polyclonal antibody

CXCL2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 16325-1-AP

Synonyms

Growth-regulated protein beta, GRO-beta(5-73), Gro-beta, CXCL 2, C-X-C motif chemokine 2

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Validation Data Gallery

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  WB analysis using 16325-1-AP

  Human lung tissue was subjected to SDS PAGE followed by western blot with 16325-1-AP (CXCL2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human lung tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 2 publications below

Product Information

16325-1-AP targets CXCL2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CXCL2 fusion protein Ag9410
• Full Name: chemokine (C-X-C motif) ligand 2
• Calculated Molecular Weight: 107 aa, 11 kDa
• Observed Molecular Weight: 9 kDa
• GenBank Accession Number: BC015753
• Gene Symbol: CXCL2
• Gene ID (NCBI): 2920
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CXCL2, also known as macrophage inflammatory protein 2-alpha (MIP2-alpha), is a member of CXC chemokine family that binds to CXC chemokine receptor 2 (CXCR2). CXCL2 is mainly produced by macrophages, endothelial cells, epithelial cells and tumor cells. CXCL2 plays important roles in various biological progresses such as angiogenesis, inflammation, immune response and cancer biological behaviors. Under inflammatory conditions in CNS, microglia and astrocytes may secrete CXCL2 as well as other chemokines, which induces chemotaxis and infiltration of circulatory neutrophils. Indeed, previous studies suggest that CXCL2 is involved in Alzheimer disease, multiple sclerosis and brain ischemia.

Publications

Species	Application	Title
mouse	WB	Int Immunopharmacol; GL13K-modified titanium regulates osteogenic differentiation via the NF-κB pathway; Authors - Yu Han
mouse	IHC	Bioorg Chem; Magnesium isoglycyrrhizinate ameliorates lipopolysaccharide-induced liver injury by upregulating autophagy and inhibiting inflammation via IL-22 expression; Authors - Xu Zhou
human	IHC	Transl Cancer Res; Correlations of AKIP1, CXCL1 and CXCL2 expressions with clinicopathological features and survival profiles in cervical cancer patients; Authors - Xiaoyan Wan