CXCL1 Recombinant antibody

CXCL1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240990E4

Cat no : 83937-3-RR

Synonyms

GRO-alpha(5-73), GRO-alpha(4-73), CXCL 1, C-X-C motif chemokine 1, C X C motif chemokine 1

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Validation Data Gallery

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  WB analysis of HUVEC using 83937-3-RR

  Untreated, LPS and Brefeldin A treated HUVEC cells were subjected to SDS PAGE followed by western blot with 83937-3-RR (CXCL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83937-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83937-3-RR against Human CXCL1 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83937-3-RR is CXCL1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CXCL1 fusion protein Ag2917
• Full Name: chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
• Calculated Molecular Weight: 8 kDa to 14 kDa
• Observed Molecular Weight: 11 kDa
• GenBank Accession Number: BC011976
• Gene Symbol: CXCL1
• Gene ID (NCBI): 2919
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CXCL1 a is a member of CXC family and also known as keratinocyte-derived chemokines (KC) or growth-related oncogene (GRO). CXCL1 is expressed by macrophages, neutrophils and epithelial cells. CXCL1 binding specifically to the CXC chemokine receptor CXCR2, is involved in fibrogenesis and angiogenesis. This protein also plays a role in inflammation and as a chemoattractant for neutrophils. CXCL1 is upregulated in some types of human cancer, including colorectal, bladder, breast, prostate and skin cancers.