CX3CL1 Monoclonal antibody
CX3CL1 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human and More (1)
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
2F5D5
Cat no : 60339-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | PC-3 cells |
Positive IHC detected in | human brain tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
IF | See 2 publications below |
Product Information
60339-1-Ig targets CX3CL1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | CX3CL1 fusion protein Ag0161 |
Full Name | chemokine (C-X3-C motif) ligand 1 |
Calculated Molecular Weight | 42 kDa |
Observed Molecular Weight | 90 kDa |
GenBank Accession Number | BC001163 |
Gene Symbol | CX3CL1 |
Gene ID (NCBI) | 6376 |
RRID | AB_2881448 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CX3CL1 antibody 60339-1-Ig | Download protocol |
IHC protocol for CX3CL1 antibody 60339-1-Ig | Download protocol |
FC protocol for CX3CL1 antibody 60339-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
JCI Insight Single-cell transcriptome analysis and protein profiling reveal broad immune system activation in IgG4-related disease | ||
Neurosci Bull Multiple Mild Stimulations Reduce Membrane Distribution of CX3CR1 Promoted by Annexin a1 in Microglia to Attenuate Excessive Dendritic Spine Pruning and Cognitive Deficits Caused by a Transient Ischemic Attack in Mice |