CX3CL1 Monoclonal antibody

CX3CL1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2F5D5

Cat no : 60339-1-Ig

Synonyms

ABCD 3, C X3 C motif chemokine 1, C3Xkine, CX3CL1, CXC3, CXC3C, FKN, fractalkine, neurotactin, NTN, NTT, SCYD1, Small inducible cytokine D1



Tested Applications

Positive WB detected inPC-3 cells
Positive IHC detected inhuman brain tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60339-1-Ig targets CX3CL1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CX3CL1 fusion protein Ag0161
Full Name chemokine (C-X3-C motif) ligand 1
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC001163
Gene Symbol CX3CL1
Gene ID (NCBI) 6376
RRIDAB_2881448
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CX3CL1 antibody 60339-1-IgDownload protocol
IHC protocol for CX3CL1 antibody 60339-1-IgDownload protocol
FC protocol for CX3CL1 antibody 60339-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

JCI Insight

Single-cell transcriptome analysis and protein profiling reveal broad immune system activation in IgG4-related disease

Authors - Chenyang Lu
mouseWB,IF

Neurosci Bull

Multiple Mild Stimulations Reduce Membrane Distribution of CX3CR1 Promoted by Annexin a1 in Microglia to Attenuate Excessive Dendritic Spine Pruning and Cognitive Deficits Caused by a Transient Ischemic Attack in Mice

Authors - Lu Zheng