CUL7 Polyclonal antibody

• Featured Product
• KD/KO Validated

CUL7 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 13738-1-AP

Synonyms

CUL 7, CUL7, cullin 7, KIAA0076

---

Validation Data Gallery

• 

  WB analysis of HEK-293 using 13738-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13738-1-AP (CUL7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13738-1-AP

  WB result of CUL7 antibody (13738-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CUL7 transfected HEK-293 cells.

---

Tested Applications

• Positive WB detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

13738-1-AP targets CUL7 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CUL7 fusion protein Ag4675
• Full Name: cullin 7
• Calculated Molecular Weight: 1698 aa, 191 kDa
• Observed Molecular Weight: 185 kDa
• GenBank Accession Number: BC033647
• Gene Symbol: CUL7
• Gene ID (NCBI): 9820
• RRID: AB_10640531
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Humans express seven cullin proeins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality. CUL7 was originally discovered as 185-kDa protein associated with the large T antigen of simian virus 40 (SV40). CUL7-deficient mice exhibit neonatal lethality with reduced size and vascular defects. CUL7 presumably plays a role in the DNA damage response by limiting p53 activity. CUL7 mutations have also been identified in 3-Msyndrome and the Yakuts short stature syndrome, both of which are characterized by pre- and post-natal growth retardation but with relatively normal mental and endocrine functions, suggesting that CUL7 may also be crucial for human placental development.

Publications

Species	Application	Title
	WB	Diabetes; Liver kinase b1 is required for white adipose tissue growth and differentiation.; Authors - Zhang Wencheng W
human	WB	J Cell Sci; Cullin-3-KCTD10-mediated CEP97 degradation promotes primary cilium formation.; Authors - Tomoaki Nagai ; KD Validated
rat	WB	Am J Physiol Renal Physiol; Ubiquitination of NKCC2 by the Cullin-RING E3 Ubiquitin Ligase Family in the Rat Thick Ascending Limb of the Loop of Henle; Authors - Gustavo R Ares