CUL4A Polyclonal antibody

• Featured Product
• KD/KO Validated

CUL4A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 10693-1-AP

Synonyms

CUL 4A, CUL4A, cullin 4A, Cullin-4A

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 10693-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10693-1-AP (CUL4A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 10693-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10693-1-AP (CUL4A antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 10693-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10693-1-AP (CUL4A antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

10693-1-AP targets CUL4A in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CUL4A fusion protein Ag1030
• Full Name: cullin 4A
• Calculated Molecular Weight: 77 kDa
• Observed Molecular Weight: 88 kDa
• GenBank Accession Number: BC008308
• Gene Symbol: CUL4A
• Gene ID (NCBI): 8451
• RRID: AB_10896455
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cullin proteins assemble a large number of RING E3 ubiquitin ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), have been identified. The two CUL4 sequences are 83% identical. They target certain proteins for degradation by binding protein DDB1 to form a CUL4-DDB1 ubiquitin ligase complex with DDB. They form two individual E3 ligases, DDB1-CUL4ADDB2 and DDB1-CUL4BDDB2 in this process. CUL4A appeared in both the nucleus and the cytosol, suggesting a more complex mechanism for entering the nucleus. CUL4B is localized in the nucleus and facilitates the transfer of DDB1 into the nucleus independently of DDB2.

Publications

Species	Application	Title
human	IHC	Breast Cancer Res; Comprehensive characterization of the DNA amplification at 13q34 in human breast cancer reveals TFDP1 and CUL4A as likely candidate target genes.; Authors - Melchor Lorenzo L
human	WB	J Cell Sci; Cullin-3-KCTD10-mediated CEP97 degradation promotes primary cilium formation.; Authors - Tomoaki Nagai ; KD Validated
human	WB	Mol Carcinog; Norcantharidin promotes cancer radiosensitization through Cullin1 neddylation-mediated CDC6 protein degradation.; Authors - Tanggang Deng ; KD Validated
mouse	WB,IF	Sci Adv; Stress-induced reduction of Na+/H+ exchanger isoform 1 promotes maladaptation of neuroplasticity and exacerbates depressive behaviors; Authors - Ye Li
human	WB	Cell Rep; Pharmacological inhibition of neddylation impairs long interspersed element 1 retrotransposition; Authors - Yan Li ; KD Validated