CUL2 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

CUL2 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A6G9

Cat no : 67175-1-PBS

Synonyms

CUL 2, CUL2, cullin 2

---

Validation Data Gallery

• 

  WB analysis of MCF-7 using 67175-1-Ig (same clone as 67175-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67175-1-Ig (CUL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 67175-1-Ig (same clone as 67175-1-PBS)

  WB result of CUL2 antibody (67175-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CUL2 transfected Raji cells. This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 67175-1-Ig (same clone as 67175-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 67175-1-Ig (CUL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 67175-1-Ig (same clone as 67175-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67175-1-Ig (CUL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67175-1-Ig (same clone as 67175-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67175-1-Ig (CUL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67175-1-Ig (same clone as 67175-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67175-1-Ig (CUL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67175-1-Ig (same clone as 67175-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67175-1-Ig (CUL2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67175-1-Ig (same clone as 67175-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67175-1-Ig (CUL2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 67175-1-Ig (same clone as 67175-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67175-1-Ig (CUL2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 67175-1-Ig (same clone as 67175-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67175-1-Ig (CUL2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67175-1-Ig (same clone as 67175-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CUL2 antibody (67175-1-Ig, Clone: 1A6G9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67175-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67175-1-PBS targets CUL2 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Rat samples.

• Tested Reactivity: Human, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CUL2 fusion protein Ag28654
• Full Name: cullin 2
• Calculated Molecular Weight: 87 kDa
• Observed Molecular Weight: 85-90 kDa
• GenBank Accession Number: BC009591
• Gene Symbol: CUL2
• Gene ID (NCBI): 8453
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Humans express seven cullin proteins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.