Cathepsin H Polyclonal antibody

Cathepsin H Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 10315-1-AP

Synonyms

CTSH, UniProtKB, Rhea, ENZYME, CPSB

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Validation Data Gallery

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  WB analysis using 10315-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10315-1-AP (Cathepsin H antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10315-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10315-1-AP (Cathepsin H antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 10315-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10315-1-AP (Cathepsin H antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 10315-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10315-1-AP (Cathepsin H antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 10315-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Cathepsin H antibody (10315-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, HaCat cells, Raji cells, U-937 cells
• Positive IHC detected in: mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 3 publications below
• FC: See 1 publications below

Product Information

10315-1-AP targets Cathepsin H in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cathepsin H fusion protein Ag0361
• Full Name: cathepsin H
• Calculated Molecular Weight: 41 kDa, 28 kDa
• Observed Molecular Weight: 28, 41 kDa
• GenBank Accession Number: BC002479
• Gene Symbol: Cathepsin H
• Gene ID (NCBI): 1512
• RRID: AB_2087534
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cathepsin H (CTSH, synonyms: CPSB, MGC1519, minichain) is a lysosomal cysteine proteinase important in the overall degradation of lysosomal proteins. It is composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. This protein, which belongs to the peptidase C1 protein family, can act both as an aminopeptidase and as an endopeptidase. Increased expression of this gene has been correlated with malignant progression of prostate tumors.Cathepsin H is a unique member of the cysteine cathepsins that acts primarily as an aminopeptidase. Like other cysteine cathepsins, it is synthesized as an inactive precursor and activated by proteolytic removal of its propeptide. In human cells, Cathepsin H exists an autocatalytic form, proceeding from an inactive 41 kDa pro-form, through a 30 kDa intermediate form, to the 28 kDa mature form (PMID: 22704610).

Publications

Species	Application	Title
human	IHC	Cell Rep Med; Distinct immune microenvironment of lung adenocarcinoma in never-smokers from smokers; Authors - Wenxin Luo
human	IHC	Oncogene; Cathepsin H regulated by the thyroid hormone receptors associate with tumor invasion in human hepatoma cells.; Authors - Wu S-M SM
rat	IHC	Microscopy (Oxf); Histochemical examination on principal collagen fibers in periodontal ligaments of ascorbic acid-deficient ODS-od/od rats.; Authors - Tomoka Hasegawa
rat	WB	Front Pediatr; Impaired FGF10 Signaling and Epithelial Development in Experimental Lung Hypoplasia With Esophageal Atresia.; Authors - Jun Wang
mouse	FC	Nature; Anti-tumour immunity controlled through mRNA m6A methylation and YTHDF1 in dendritic cells.; Authors - Dali Han
mouse	WB	J Inflamm Res; Identifying and Validating Extracellular Matrix-Related Gene CTSH in Diabetic Foot Ulcer Using Bioinformatics and Machine Learning; Authors - Pei-Yu Wu