Cathepsin G Polyclonal antibody

Cathepsin G Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 23840-1-AP

Synonyms

Cathepsin G, CG, CTSG

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Validation Data Gallery

• 

  WB analysis of MCF-7 using 23840-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 23840-1-AP (Cathepsin G antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-453s using 23840-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 23840-1-AP (Cathepsin G antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: MCF-7 cells, MDA-MB-453s cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

The immunogen of 23840-1-AP is Cathepsin G Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cathepsin G fusion protein Ag20818
• Full Name: cathepsin G
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 29 kDa, 35 kDa
• GenBank Accession Number: BC014460
• Gene Symbol: Cathepsin G
• Gene ID (NCBI): 1511
• RRID: AB_2879335
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTSG(Cathepsin G) is also named as CTLA, CG, MGC23078, Catg and belongs to the peptidase S1 family. It may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation. CTSG may also play a role in the progression to heart failure by activating angiotensin II, leading to detrimental effects on the heart. The full length protein contains 255 amino acid and has a signal peptide, a propeptide and a glycosylation site.

Publications

Species	Application	Title
human	WB	Cancer Cell; Cathepsin C promotes breast cancer lung metastasis by modulating neutrophil infiltration and neutrophil extracellular trap formation.; Authors - Yansen Xiao
human	WB	Front Immunol; A Novel Immune-Related Gene Signature to Identify the Tumor Microenvironment and Prognose Disease Among Patients With Oral Squamous Cell Carcinoma Patients Using ssGSEA: A Bioinformatics and Biological Validation Study; Authors - Yun Chen
		PLoS One; Stable depletion of RUNX1-ETO in Kasumi-1 cells induces expression and enhanced proteolytic activity of Cathepsin G and Neutrophil Elastase.; Authors - Caroline Schoenherr