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Tested Applications
| Positive IHC detected in | human prostate cancer tissue, human heart tissue, human stomach cancer tissue, mouse heart tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 1 publications below |
| IHC | See 3 publications below |
Product Information
11055-1-AP targets Cathepsin F in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Cathepsin F fusion protein Ag1521 Predict reactive species |
| Full Name | cathepsin F |
| Calculated Molecular Weight | 53 kDa and 34 kDa |
| GenBank Accession Number | BC011682 |
| Gene Symbol | Cathepsin F |
| Gene ID (NCBI) | 8722 |
| RRID | AB_2292434 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UBX1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CTSF(Cathepsin F) is a member of the papain family of cysteine proteases. These enzymes represent a major component of the lysosomal proteolytic system. They are synthesized as inactive precursors consisting of a signal sequence, a propeptide, and a catalytically active mature region. Cathepsins are routed to the endosomal/lysosomal compartment via the mannose 6-phosphate receptor pathway. The deduced 484-amino acid cathepsin F protein has a signal sequence and potential glycosylation sites.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Cathepsin F antibody 11055-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Stem Cell Res Ther Adipose-derived stem cells alleviate radiation-induced dermatitis by suppressing apoptosis and downregulating cathepsin F expression.
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Brain Res Bull Adenovirus-driven overexpression of proteinases in organ-cultured normal human corneas leads to diabetic-like changes. | ||
Proc Natl Acad Sci U S A A small-molecule drug inhibits autophagy gene expression through the central regulator TFEB | ||
Oncol Res Cathepsin F Knockdown Induces Proliferation and Inhibits Apoptosis in Gastric Cancer Cells
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