CTRP1 Polyclonal antibody

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  WB analysis using 30289-1-AP

  human adipose tissue was subjected to SDS PAGE followed by western blot with 30289-1-AP (CTRP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 30289-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 30289-1-AP (CTRP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 30289-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30289-1-AP (CTRP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CTRP1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 30289-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

C1QTNF1, Complement C1q tumor necrosis factor-related protein 1, G protein-coupled receptor-interacting protein, GIP, UNQ310/PRO353

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Tested Applications

• Positive WB detected in: human adipose tissue
• Positive IHC detected in: mouse heart tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30289-1-AP targets CTRP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CTRP1 fusion protein Ag31779
• Full Name: C1q and tumor necrosis factor related protein 1
• Calculated Molecular Weight: 32KD
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: NM_030968
• Gene Symbol: CTRP1
• Gene ID (NCBI): 114897
• RRID: AB_3669708
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BXJ1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C1q and tumor necrosis factor related protein 1(CTRP1), also known as C1QTNF1, is a member of the CTRP superfamily. CTRP1 is a secreted glycoprotein synthesized by a variety of tissues and cells, such as adipose tissue and macrophages. CTRP1 is a paralog of adiponectin. CTRP1 plays a critical role in regulating inflammation and lipid metabolism and acts as a targeted treatment for atherosclerosis, heart failure, hypertension, NAFLD, and diabetes mellitus (PMID: 36812667).