CTRL Polyclonal antibody, PBS Only

CTRL Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14856-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of mouse pancreas using 14856-1-AP (same clone as 14856-1-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14856-1-AP (CTRL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14856-1-PBS in a different storage buffer formulation.

• 

  WB analysis of BxPC-3 using 14856-1-AP (same clone as 14856-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14856-1-AP (CTRL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14856-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse pancreas using 14856-1-AP (same clone as 14856-1-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14856-1-AP (CTRL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14856-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 14856-1-AP (same clone as 14856-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 14856-1-AP (CTRL antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14856-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 14856-1-AP (same clone as 14856-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 14856-1-AP (CTRL antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14856-1-PBS in a different storage buffer formulation.

• 

  IF Staining of BxPC-3 using 14856-1-AP (same clone as 14856-1-PBS)

  Immunofluorescent analysis of BxPC-3 cells using 14856-1-AP (CTRL antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14856-1-PBS in a different storage buffer formulation.

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Product Information

14856-1-PBS targets CTRL in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CTRL fusion protein Ag6644
• Full Name: chymotrypsin-like
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 25-28 kDa
• GenBank Accession Number: BC063475
• Gene Symbol: CTRL
• Gene ID (NCBI): 1506
• RRID: AB_10640303
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CTRL(Chymotrypsin-like protease) is also named as CTRL1 and belongs to the peptidase S1 family. The CTRL1 enzyme is active over a broad pH range. It expresses in pancreas and presence in intestinal fluid after stimulation of pancreatic secretion as a digestive enzyme(PMID:9065485). This gene encode a protein of 264 amino acid and the protein has a signal peptide of 18 amino acid and a propeptide of 15 amino acid.