p120 Catenin Polyclonal antibody

• Featured Product
• KD/KO Validated

p120 Catenin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 12180-1-AP

Synonyms

CTNND1, CTNND, Catenin delta-1, Catenin delta 1, CAS

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Validation Data Gallery

• 

  WB analysis of human brain using 12180-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12180-1-AP (p120 Catenin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12180-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12180-1-AP (p120 Catenin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 12180-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12180-1-AP (p120 Catenin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 12180-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 12180-1-AP (p120 Catenin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12180-1-AP

  WB result of p120 Catenin antibody (12180-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p120 Catenin transfected HeLa cells.

• 

  WB analysis of HEK-293 using 12180-1-AP

  WB result of p120 Catenin antibody (12180-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p120 Catenin transfected HEK-293 cells.

• 

  WB analysis of human brain using 12180-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12180-1-AP (p120 Catenin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12180-1-AP

  IP result of anti-p120 Catenin (IP:12180-1-AP, 4ug; Detection:12180-1-AP 1:500) with mouse brain tissue lysate 2640ug.

• 

  IHC staining of human breast cancer using 12180-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12180-1-AP (p120 Catenin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12180-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12180-1-AP (p120 Catenin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12180-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12180-1-AP (p120 Catenin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12180-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12180-1-AP (p120 Catenin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 12180-1-AP

  Immunofluorescent analysis of HepG2 cells, using CTNND1 antibody 12180-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: human brain tissue, HeLa cells, HEK-293 cells, mouse brain tissue, NIH/3T3 cells, RAW264.7 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human breast cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 15 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

12180-1-AP targets p120 Catenin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: p120 Catenin fusion protein Ag2824
• Full Name: catenin (cadherin-associated protein), delta 1
• Calculated Molecular Weight: 948 aa, 105 kDa
• Observed Molecular Weight: 90-120 kDa
• GenBank Accession Number: BC010501
• Gene Symbol: p120 Catenin
• Gene ID (NCBI): 1500
• RRID: AB_2086267
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Catenins were discovered as proteins that are linked to the cytoplasmic domain of transmembrane cadherins (PMID: 9653641). p120 catenin, also called p120 ctn or catenin delta-1, regulates cell-cell adhesion through its interaction with the cytoplasmic tail of classical and type II cadherins. p120 catenin is a tyrosine kinase substrate implicated in cell transformation by SRC, as well as in ligand-induced receptor signaling through the EGF receptor, the PDGF receptor, and the CSF1 receptor. Different expression patterns of p120 catenin in lobular and ductal carcinomas of breast have been reported: membrane stain for ductal carcinoma and cytoplasmic stain for lobular carcinoma (PMID: 24966968). Different isoforms of p120 catenin are variably expressed in different tissues as a result of alternative splicing and the use of multiple translation initiation codons (PMID: 19150613).

Publications

Species	Application	Title
mouse	WB	Nat Hum Behav; Genome-wide meta-analysis, functional genomics and integrative analyses implicate new risk genes and therapeutic targets for anxiety disorders; Authors - Wenqiang Li
mouse	WB	EMBO Mol Med; Inhibition of Sema4D/PlexinB1 signaling alleviates vascular dysfunction in diabetic retinopathy.; Authors - Jie-Hong Wu
mouse	WB	EBioMedicine; NDRG2 regulates adherens junction integrity to restrict colitis and tumourigenesis.; Authors - Mengying Wei
human	WB	Cell Cycle; Hsa-miR-425-5p promotes tumor growth and metastasis by activating the CTNND1-mediated β-catenin pathway and EMT in colorectal cancer.; Authors - Dingsheng Liu
human,mouse	WB	Front Pharmacol; ALG-bFGF Hydrogel Inhibiting Autophagy Contributes to Protection of Blood-Spinal Cord Barrier Integrity via PI3K/Akt/FOXO1/KLF4 Pathway After SCI.; Authors - Renkan Zhang
mouse	WB	Front Mol Neurosci; F-Actin Dysplasia Involved in Organ of Corti Deformity in Gjb2 Knockdown Mouse Model.; Authors - Xiao-Zhou Liu

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