SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14362-1-AP (alpha N Catenin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14362-1-AP (alpha N Catenin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 14362-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14362-1-AP (alpha N Catenin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14362-1-AP (alpha N Catenin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IP experiment of SH-SY5Y using 14362-1-AP
IP result of anti-Alpha N-Catenin (IP:14362-1-AP, 4ug; Detection:14362-1-AP 1:500) with SH-SY5Y cells lysate 1600ug.
IP result of anti-Alpha N-Catenin (IP:14362-1-AP, 4ug; Detection:14362-1-AP 1:500) with SH-SY5Y cells lysate 1600ug.
IHC staining of rat brain using 14362-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14362-1-AP (Alpha N-Catenin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14362-1-AP (Alpha N-Catenin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 14362-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14362-1-AP (Alpha N-Catenin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14362-1-AP (Alpha N-Catenin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of SH-SY5Y using 14362-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Alpha N-Catenin antibody (14362-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Alpha N-Catenin antibody (14362-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
SH-SY5Y cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:3000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14362-1-AP (alpha N Catenin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 14362-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14362-1-AP (alpha N Catenin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of rat brain using 14362-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14362-1-AP (Alpha N-Catenin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 14362-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14362-1-AP (Alpha N-Catenin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of SH-SY5Y using 14362-1-AP
IP result of anti-Alpha N-Catenin (IP:14362-1-AP, 4ug; Detection:14362-1-AP 1:500) with SH-SY5Y cells lysate 1600ug.
IF/ICC Figures
IF Staining of SH-SY5Y using 14362-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Alpha N-Catenin antibody (14362-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
Alpha N-Catenin Polyclonal antibody
Catalog Number
14362-1-AP
Citations
2
Dilutions
WB : 1:500-1:3000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:500-1:2000 IF/ICC : 1:200-1:800
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
