Anti-Human CTLA-4/CD152 Rabbit Recombinant Antibody

CTLA-4/CD152 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240793E8

Cat no : 98078-1-RR

Synonyms

CTLA4, Cytotoxic T-lymphocyte protein 4, CTLA 4, CELIAC3, CD152

---

Validation Data Gallery

• 

  FC experiment of human PBMCs using 98078-1-RR

  1x10^6 PHA stimulated human PBMCs were surface stained with 0.25 ug Anti-Human CTLA4 Rabbit Recombinant Antibody (98078-1-RR, Clone:240793E8) , or 1x10^6 unstimulated human PBMCs were surface stained with 0.25 ug Anti-Human CTLA4 Rabbit Recombinant Antibody (98078-1-RR, Clone:240793E8) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD3. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98078-1-RR

  1x10^6 PHA stimulated human PBMCs were surface stained with 0.25 ug Anti-Human CTLA4 Rabbit Recombinant Antibody (98078-1-RR, Clone:240793E8)(red), or Isotype Control(blue), or 1x10^6 unstimulated human PBMCs were surface stained with 0.25 ug Anti-Human CTLA4 Rabbit Recombinant Antibody (98078-1-RR, Clone:240793E8)(black) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) . Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98078-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98078-1-RR against Human CTLA4/CD152 were performed. The affinity constant is 60.2 pM.

---

Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98078-1-RR is CTLA-4/CD152 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: cytotoxic T-lymphocyte-associated protein 4
• Calculated Molecular Weight: 223 aa, 25 kDa
• GenBank Accession Number: BC070162
• Gene Symbol: CTLA4
• Gene ID (NCBI): 1493
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.