CTLA-4/CD152 Polyclonal antibody

CTLA-4/CD152 Polyclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 30648-1-AP

Synonyms

CTLA-4, Ctla4, Ly 56, Ctla 4, Cd152

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Validation Data Gallery

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  WB analysis using 30648-1-AP

  Untreated or Con A-treated (3 ug/ml, 72h) mouse splenocytes were subjected to SDS PAGE followed by western blot with 30648-1-AP (CTLA-4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse spleen using 30648-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CTLA-4 antibody (30648-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 CD3 antibody (CL594-60181, Clone: 3F3A1, red).

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Tested Applications

• Positive WB detected in: Con A treated mouse splenocytes
• Positive IF-P detected in: mouse spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

30648-1-AP targets CTLA-4/CD152 in WB, IF-P, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: cytotoxic T-lymphocyte-associated protein 4
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 28-40 kDa
• GenBank Accession Number: NM_009843.4
• Gene Symbol: CTLA-4
• Gene ID (NCBI): 12477
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTLA-4, also known as CD152, belonging to the immunoglobulin superfamily, is primarily found on activated T cells and regulatory T cells (Tregs). CTLA-4 is closely related to the T-cell costimulatory CD28, and both molecules bind to B7-1 and B7-2 on antigen-presenting cells. CTLA-4 acts as a negative regulatory molecule of T-cell responses. Besides the full-length transmembrane form, CTLA-4 also exists in a truncated soluble form (sCTLA-4).

Publications

Species	Application	Title
	WB	Int Immunopharmacol; Chemokine‑ and chemokine receptor-based subtypes predict prognosis, immunotherapy and chemotherapy response in colorectal cancer patients; Authors - Wenjie Zhu