Gamma Cystathionase Polyclonal antibody

• Featured Product
• KD/KO Validated

Gamma Cystathionase Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA, Cell treatment

Conjugate

Unconjugated

Publications

199

Reviews (1)

Cat no : 12217-1-AP

Synonyms

CSE, CGL, EC:4.4.1.2, EC:4.4.1.1, Cystathionine gamma-lyase

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Validation Data Gallery

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  WB analysis using 12217-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12217-1-AP (Gamma Cystathionase antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 cells using 12217-1-AP

  WB result of CTH antibody (12217-1-AP, 1:500) with si-control and si-CTH transfected HepG2 cell.

• 

  WB analysis of HeLa using 12217-1-AP

  WB result of Gamma cystathionase antibody (12217-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Gamma cystathionase transfected HeLa cells.

• 

  WB analysis of mouse heart using 12217-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12217-1-AP (Gamma cystathionase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 12217-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 12217-1-AP (Gamma cystathionase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12217-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12217-1-AP (Gamma cystathionase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12217-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12217-1-AP (Gamma Cystathionase antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 12217-1-AP

  IP result of anti-Gamma Cystathionase (IP:12217-1-AP, 4ug; Detection:12217-1-AP 1:8000) with mouse liver tissue lysate 1840 ug.

• 

  IHC staining of human liver cancer using 12217-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12217-1-AP (Gamma cystathionase antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12217-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12217-1-AP (Gamma cystathionase antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 12217-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12217-1-AP (Gamma cystathionase antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 12217-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12217-1-AP (Gamma cystathionase antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 12217-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12217-1-AP (Gamma cystathionase antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12217-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12217-1-AP (Gamma cystathionase antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12217-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12217-1-AP (Gamma cystathionase antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of human liver cancer using 12217-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Gamma Cystathionase antibody (12217-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 12217-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Gamma Cystathionase antibody (12217-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of MCF-7 using 12217-1-AP

  1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug Gamma Cystathionase Polyclonal antibody (12217-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, mouse heart tissue, mouse kidney tissue, mouse liver tissue, rat heart tissue, rat liver tissue, rat kidney tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human liver cancer tissue, human breast cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human liver cancer tissue
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:500
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 20 publications below
• WB: See 174 publications below
• IHC: See 28 publications below
• IF: See 12 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

12217-1-AP targets Gamma Cystathionase in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Gamma Cystathionase fusion protein Ag2872
• Full Name: cystathionase (cystathionine gamma-lyase)
• Calculated Molecular Weight: 405 aa, 45 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC015807
• Gene Symbol: Gamma Cystathionase
• Gene ID (NCBI): 1491
• RRID: AB_2087497
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTH, also named as Gamma-cystathionase and CSE, belongs to the transsulfuration enzymes family. It catalyzes the last step in the transsulfuration pathway from methionine to cysteine. CTH converts two cysteine molecules to lanthionine and hydrogen sulfide. CTH can also accept homocysteine as substrate. It specificity depends on the levels of the endogenous substrates. CTH is the major H2S-producing enzyme in kidney, liver, vascular smooth muscle cells and enterocytes. The endogenous production of H2S plays a significant role in the regulation of cellular functions, including cell growth, hyperpolarization of cell membranes, modulation of neuronal excitability and relaxation of smooth muscle cells. The CSE/H2S pathway is upregulated in the heart in a murine model of CVB3-induced myocarditis and that inhibition of endogenous H2S is beneficial to treatment early in the disease while administration of exogenous H2S is protective to infected myocardium during the later stage. Mutations in the gene encoding CTH can result in the autosomal recessive disease cystathioninuria; a disorder characterized by the unusual accumulation of plasma cystathionine causing increased urinary excretion. Both male and female CTH-null mice showed hypercystathioninemia and hyperhomocysteinemia, but not hypermethioninemia. CSE has also been reported to be expressed in endothelial cells and contribute to endothelium-dependent vasorelaxation. It can be detected a minor 36 kDa band probably representing a degradative intermediate except the major 43 kDa band in vitamin B6-deficient rat liver(PMID:8660672). CTH also can be detected as ~70kD in rat liver (PMID: 18974309). This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human CTH.

Publications

Species	Application	Title
human	Cell treatment	Cell Metab; Microenvironmental ammonia enhances T cell exhaustion in colorectal cancer; Authors - Hannah N Bell
human	WB	Cell Metab; Transsulfuration Activity Can Support Cell Growth upon Extracellular Cysteine Limitation.; Authors - Jiajun Zhu
mouse	WB	Cell Metab; Fibroblast Growth Factor 21 Drives Dynamics of Local and Systemic Stress Responses in Mitochondrial Myopathy with mtDNA Deletions.; Authors - Saara Forsström
mouse	WB	Cell Metab; mTORC1 Regulates Mitochondrial Integrated Stress Response and Mitochondrial Myopathy Progression.; Authors - Nahid A Khan
	WB	Cell Metab; Mitochondrial DNA Replication Defects Disturb Cellular dNTP Pools and Remodel One-Carbon Metabolism.; Authors - Joni Nikkanen
human	WB	Mol Cell; mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.; Authors - Elodie Villa

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Piruthivi (Verified Customer) (12-09-2019)

Western blot was very clear.

• Applications: Western Blot,

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