CTGF Polyclonal antibody

CTGF Polyclonal Antibody for WB, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (5)

Applications

WB, IHC, IF, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

32

Cat no : 25474-1-AP

Synonyms

IBP 8, Hypertrophic chondrocyte-specific protein 24, HCS24, Cellular communication network factor 2, CCN2

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Validation Data Gallery

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  WB analysis using 25474-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25474-1-AP (CTGF antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 25474-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25474-1-AP (CTGF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HUVEC using 25474-1-AP

  IP result of anti-CTGF (IP:25474-1-AP, 4ug; Detection:25474-1-AP 1:1000) with HUVEC cells lysate 1440 ug.

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  FC experiment of HuH-7 using 25474-1-AP

  1X10^6 HuH-7 cells were intracellularly stained with 0.4 ug Anti-Human CTGF (25474-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HUVEC cells, HeLa cells, U2OS cells
• Positive IP detected in: HUVEC cells
• Positive FC (Intra) detected in: HuH-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 31 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Product Information

25474-1-AP targets CTGF in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, pig, canine, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: connective tissue growth factor
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: NM_001901
• Gene Symbol: CTGF
• Gene ID (NCBI): 1490
• RRID: AB_2918089
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTGF, also known as CCN2, is a member of the CCN family of matricellular proteins. CTGF, a cysteine-rich, matrix-associated, heparin-binding protein, is widely expressed in various human tissues and organs. CTGF has important roles in many biological processes, including cell adhesion, migration, proliferation, angiogenesis, skeletal development, and tissue wound repair, and is critically involved in fibrotic disease and several forms of cancers.

Publications

Species	Application	Title
human	WB	Oxid Med Cell Longev; Cigarette Smoke Exposure Increases Glucose-6-phosphate Dehydrogenase, Autophagy, Fibrosis, and Senescence in Kidney Cells In Vitro and In Vivo.; Authors - Wen-Chih Liu
pig	WB	Front Bioeng Biotechnol; Effect of stem cell conditional medium-loading adhesive hydrogel on TGF-β1-induced endometrial stromal cell fibrosis; Authors - Yuan Zhu
mouse	WB	Eur J Pharmacol; Palmatine alleviates cardiac fibrosis by inhibiting fibroblast activation through the STAT3 pathway; Authors - Shaoling Lin
	WB	PLoS Negl Trop Dis; Mining host candidate regulators of schistosomiasis-induced liver fibrosis in response to artesunate therapy through transcriptomics approach; Authors - Yajie Yuan
mouse	WB	J Mol Histol; METTL3 silencing inhibits ferroptosis to suppress ovarian fibrosis in PCOS by upregulating m6A modification of GPX4; Authors - Chuan Shen
rat	WB	Chin Med; Downregulation of lncRNA Miat contributes to the protective effect of electroacupuncture against myocardial fibrosis.; Authors - Wenchuan Qi