CTGF Polyclonal antibody

CTGF Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 23903-1-AP

Synonyms

CTGF

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Validation Data Gallery

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  WB analysis using 23903-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23903-1-AP (CTGF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 23903-1-AP

  IP result of anti-CTGF (IP:23903-1-AP, 4ug; Detection:23903-1-AP 1:1000) with mouse brain tissue lysate 1800 ug.

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  IHC staining of mouse brain using 23903-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23903-1-AP (CTGF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 23903-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 23903-1-AP (CTGF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 23903-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23903-1-AP (CTGF Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human nephroblastoma using 23903-1-AP

  Immunohistochemical analysis of paraffin-embedded human nephroblastoma tissue slide using 23903-1-AP (CTGF Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse heart tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse heart tissue, human heart tissue, human nephroblastoma tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 12 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Product Information

23903-1-AP targets CTGF in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CTGF fusion protein Ag20985
• Full Name: connective tissue growth factor
• Calculated Molecular Weight: 349 aa, 38 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC087839
• Gene Symbol: CTGF
• Gene ID (NCBI): 1490
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTGF, also known as CCN2, is a member of the CCN family of matricellular proteins. CTGF, a cysteine-rich, matrix-associated, heparin-binding protein, is widely expressed in various human tissues and organs. CTGF has important roles in many biological processes, including cell adhesion, migration, proliferation, angiogenesis, skeletal development, and tissue wound repair, and is critically involved in fibrotic disease and several forms of cancers. In western blotting, there are three different forms of CTGF reported: monomeric forms at approximately 36-38 kDa, homodimeric forms at approximately 70 kDa, and lower molecular mass fragment forms (PMID: 14592956).

Publications

Species	Application	Title
mouse	WB	Phytomedicine; Aloe-emodin ameliorated MI-induced cardiac remodeling in mice via inhibiting TGF-β/SMAD signaling via up-regulating SMAD7; Authors - Jie Yu
mouse	WB,IHC	Biomater Sci; A high-density collagen xerogel thread prevents the progression of peritoneal fibrosis; Authors - Shigehisa Aoki
human,mouse	WB	Ecotoxicol Environ Saf; Polystyrene microplastic-induced extracellular vesicles cause kidney-related effects in the crosstalk between tubular cells and fibroblasts; Authors - Yung-Li Wang
human,rat	WB,IF	Stem Cells Int; hUMSCs Restore Uterine Function by Inhibiting Endometrial Fibrosis via Regulation of the MMP-9/TIMP-1 Ratio in CDDP-Induced Injury Rats; Authors - Yu Tang
rat	WB,IHC	J Cardiovasc Transl Res; Exosomes Derived from AT2R-Overexpressing BMSC Prevent Restenosis After Carotid Artery Injury by Attenuating the Injury-Induced Neointimal Hyperplasia.; Authors - Xinliang Zou
mouse	WB	Tissue Cell; Estrogen inhibits the differentiation of fibroblasts induced by high stiffness matrix by enhancing DNMT1 expression; Authors - Zhihan Zhao