Filter: AllWBIPIHCIF/ICC

WB analysis of MCF-7 using 17532-1-AP (same clone as 17532-1-PBS)
MCF7 cells were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.
WB analysis of mouse testis using 17532-1-AP (same clone as 17532-1-PBS)
mouse testis tissue were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 17532-1-AP (same clone as 17532-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.
WB analysis of Y79 using 17532-1-AP (same clone as 17532-1-PBS)
Y79 cells were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 17532-1-AP (same clone as 17532-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.
WB analysis of human brain using 17532-1-AP (same clone as 17532-1-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 17532-1-AP (same clone as 17532-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.
WB analysis of mouse eye using 17532-1-AP (same clone as 17532-1-PBS)
mouse eye tissue were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.
IP experiment of mouse testis using 17532-1-AP (same clone as 17532-1-PBS)
IP result of anti-CTDSPL2 (IP:17532-1-AP, 4ug; Detection:17532-1-AP 1:500) with mouse testis tissue lysate 4400ug. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.
IHC staining of human endometrial cancer using 17532-1-AP (same clone as 17532-1-PBS)
Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 17532-1-AP (CTDSPL2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 17532-1-AP (same clone as 17532-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17532-1-AP (CTDSPL2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.
IHC staining of human endometrial cancer using 17532-1-AP (same clone as 17532-1-PBS)
Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 17532-1-AP (CTDSPL2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 17532-1-AP (same clone as 17532-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17532-1-AP (CTDSPL2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 17532-1-AP (same clone as 17532-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CTDSPL2 antibody (17532-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

CTDSPL2 Polyclonal antibody, PBS Only

CTDSPL2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 17532-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

HSPC129, HSPC058, EC:3.1.3.-, CTDSP-like 2, CTDSP like 2

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

17532-1-PBS targets CTDSPL2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CTDSPL2 fusion protein Ag11581 Predict reactive species
Full Name	CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase like 2
Calculated Molecular Weight	466 aa, 53 kDa
Observed Molecular Weight	67 kDa
GenBank Accession Number	BC035744
Gene Symbol	CTDSPL2
Gene ID (NCBI)	51496
RRID	AB_2087204
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q05D32
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

CTD small phosphatase-like protein 2 (CTDSPL2) is also named as HSPC058 and HSPC129, belongs to the CTDSPL2 family. It is a putative RNA-polymerase II carboxy-terminal domain (CTD) phosphatase, whose specific CTD phosphatase activity was verified in vitro, and may regulate the dynamic phosphorylation of RNAP II CTD.( PMID: 17487459) It can obviously improve the expression of ε- and γ-globin genes in K562 cells and CD34+ cells derived from UCB.( PMID: 20932329) This antibody is a rabbit polyclonal antibody raised against a region of human CTDSPL2.