CTDSPL2 Polyclonal antibody, PBS Only

CTDSPL2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 17532-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of MCF-7 using 17532-1-AP (same clone as 17532-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 17532-1-AP (same clone as 17532-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse eye using 17532-1-AP (same clone as 17532-1-PBS)

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 17532-1-AP (same clone as 17532-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 17532-1-AP (same clone as 17532-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 17532-1-AP (same clone as 17532-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Y79 using 17532-1-AP (same clone as 17532-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 17532-1-AP (same clone as 17532-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse testis using 17532-1-AP (same clone as 17532-1-PBS)

  IP result of anti-CTDSPL2 (IP:17532-1-AP, 4ug; Detection:17532-1-AP 1:500) with mouse testis tissue lysate 4400ug. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human endometrial cancer using 17532-1-AP (same clone as 17532-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 17532-1-AP (CTDSPL2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 17532-1-AP (same clone as 17532-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17532-1-AP (CTDSPL2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human endometrial cancer using 17532-1-AP (same clone as 17532-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 17532-1-AP (CTDSPL2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 17532-1-AP (same clone as 17532-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17532-1-AP (CTDSPL2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 17532-1-AP (same clone as 17532-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 17532-1-AP (CTDSPL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

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Product Information

17532-1-PBS targets CTDSPL2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CTDSPL2 fusion protein Ag11581
• Full Name: CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase like 2
• Calculated Molecular Weight: 466 aa, 53 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC035744
• Gene Symbol: CTDSPL2
• Gene ID (NCBI): 51496
• RRID: AB_2087204
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CTD small phosphatase-like protein 2 (CTDSPL2) is also named as HSPC058 and HSPC129, belongs to the CTDSPL2 family. It is a putative RNA-polymerase II carboxy-terminal domain (CTD) phosphatase, whose specific CTD phosphatase activity was verified in vitro, and may regulate the dynamic phosphorylation of RNAP II CTD.( PMID: 17487459) It can obviously improve the expression of ε- and γ-globin genes in K562 cells and CD34+ cells derived from UCB.( PMID: 20932329) This antibody is a rabbit polyclonal antibody raised against a region of human CTDSPL2.