CSTF1 Polyclonal antibody

CSTF1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 10064-2-AP

Synonyms

CF 1 50 kDa subunit, Cleavage stimulation factor, CstF 50, CSTF 50 kDa subunit, CSTF1, CstFp50

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Validation Data Gallery

• 

  WB analysis using 10064-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10064-2-AP (CSTF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 10064-2-AP

  IP result of anti-CSTF1 (IP:10064-2-AP, 2ug; Detection:10064-2-AP 1:500) with NIH/3T3 cells lysate 2500ug.

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells
• Positive IP detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

10064-2-AP targets CSTF1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CSTF1 fusion protein Ag0109
• Full Name: cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa
• Calculated Molecular Weight: 48.3 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC001011
• Gene Symbol: CSTF1
• Gene ID (NCBI): 1477
• RRID: AB_2261145
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cleavage stimulation factor (CstF) , a heterotrimeric protein with subunits of 77, 64, and 50 kD (CstF-77, -64, and -50), is a polyadenylation factor that helps to specify the site of processing. CstF recognizes the G+U-rich element, a sequence located downstream of the cleavage site. CstF-50 subunit interacts with the COOH-terminal domain of the RNAP II largest subunit (CTD). This subunit may be responsible for the interaction of CSTF with other factors to form a satable complex on the pre-mRNA, and multiple WD domains are required for protein interactions.

Publications

Species	Application	Title
human	WB	EMBO J; The 3' ends of human pre-snRNAs are produced by RNA polymerase II CTD-dependent RNA processing.; Authors - Patricia Uguen
human	WB	Cell Rep; LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation; Authors - Nasha Zhang
human	WB	Oncogene; p53 inhibits mRNA 3' processing through its interaction with the CstF/BARD1 complex.; Authors - F I Nazeer
		Andrology; Nicotine elevates sperm motility and induces Pfn1 promoter hypomethylation in mouse testis.; Authors - J Dai
rat,mouse,human		iScience; Chromodomain helicase DNA-binding domain 2 maintains spermatogonial self-renewal by promoting chromatin accessibility and mRNA stability; Authors - Ziqian Min
human	WB	Cancer Lett; YTHDF1 regulates GID8-mediated glutamine metabolism to promote colorectal cancer progression in a m6A-dependent manner; Authors - Yicun Han

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