Cystatin C Polyclonal antibody

• Featured Product
• KD/KO Validated

Cystatin C Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

34

Cat no : 12245-1-AP

Synonyms

CST3, Post-gamma-globulin, Gamma-trace, Cystatin-C, Cystatin-3

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Validation Data Gallery

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  WB analysis using 12245-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12245-1-AP (Cystatin C antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12245-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12245-1-AP (Cystatin C antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12245-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12245-1-AP (Cystatin C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12245-1-AP

  WB result of Cystatin C antibody (12245-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cystatin C transfected HepG2 cells.

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  WB analysis of human brain using 12245-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12245-1-AP (CST3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 12245-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12245-1-AP (Cystatin C Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of Caco-2 using 12245-1-AP

  IP result of anti-Cystatin C (IP:12245-1-AP, 4ug; Detection:12245-1-AP 1:1000) with Caco-2 cells lysate 1920 ug.

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  IHC staining of human kidney using 12245-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12245-1-AP (Cystatin C Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 12245-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12245-1-AP (Cystatin C Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse kidney using 12245-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12245-1-AP (Cystatin C antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 12245-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12245-1-AP (Cystatin C antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 12245-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 12245-1-AP (Cystatin C antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary using 12245-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 12245-1-AP (Cystatin C Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human ovary using 12245-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 12245-1-AP (Cystatin C Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of mouse kidney using 12245-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using Cystatin C antibody (12245-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse kidney using 12245-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using Cystatin C antibody (12245-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 12245-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 12245-1-AP (Cystatin C antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Caco-2 cells, human brain tissue, fetal human brain tissue, human milk, HepG2 cells, mouse brain tissue, HeLa cells, U-87 MG cells, U2OS cells, human urine, human serum
• Positive IP detected in: Caco-2 cells
• Positive IHC detected in: human kidney tissue, human ovary tissue, human ovary cancer tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse kidney tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 30 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

12245-1-AP targets Cystatin C in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cystatin C fusion protein Ag2890
• Full Name: cystatin C
• Calculated Molecular Weight: 146 aa, 16 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC013083
• Gene Symbol: Cystatin C
• Gene ID (NCBI): 1471
• RRID: AB_2088058
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cystatin C is a 13-kDa inhibitor of cysteine proteinases which is secreted by all cell types and is completely cleared from the organism through glomerular filtration, shown to be an early and sensitive biomarker of renal dysfunction. It is also used as an emerging biomarker in cardiovascular disease. Cystatin C is involved in a variety of inflammatory reactions. The concentration of serum cystatin C has also been shown to be unaltered in certain inflammatory conditions or other disorders of metabolism. The plasma level of serum cystatin C can be expressed as its level of generation from cells and diet and its subsequent elimination through the gut, liver, and kidneys.

Publications

Species	Application	Title
pig	WB	Biosens Bioelectron; Microneedle patches integrated with lateral flow cassettes for blood-free chronic kidney disease point-of-care testing during a pandemic.; Authors - Yi-Jyun Chen
human	WB,IF	Environ Pollut; Insight into the negative impact of ionic liquid: A cytotoxicity mechanism of 1-methyl-3-octylimidazolium bromide.; Authors - Junguo Ma
human	WB	J Hazard Mater; Graphene oxide disrupted mitochondrial homeostasis through inducing intracellular redox deviation and autophagy-lysosomal network dysfunction in SH-SY5Y cells.; Authors - Feng Xiaoli
rat	WB	Cell Biol Toxicol; Inhibition of ER stress attenuates kidney injury and apoptosis induced by 3-MCPD via regulating mitochondrial fission/fusion and Ca2+ homeostasis.; Authors - Yujie Zhong
human, rat	WB	Mol Nutr Food Res; Diosgenin Protects Against Kidney Injury and Mitochondrial Apoptosis Induced by 3-MCPD Through the Regulation of ER Stress, Ca2+ Homeostasis, and Bcl2 Expression.; Authors - Yujie Zhong
rat	WB	J Cell Mol Med; LINC00963 targeting miR-128-3p promotes acute kidney injury process by activating JAK2/STAT1 pathway.; Authors - Li-Bo Xie