CSRP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

CSRP2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 10892-2-AP

Synonyms

CRP2, CSRP2, Cysteine rich protein 2, LIM domain only protein 5, LMO 5, LMO5, SmLIM, Smooth muscle cell LIM protein

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Validation Data Gallery

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  WB analysis using 10892-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10892-2-AP (CSRP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 10892-2-AP

  IP result of anti-CSRP2 (IP:10892-2-AP, 4ug; Detection:10892-2-AP 1:1000) with mouse liver tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse kidney tissue, rat kidney tissue
• Positive IP detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

10892-2-AP targets CSRP2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CSRP2 fusion protein Ag1338
• Full Name: cysteine and glycine-rich protein 2
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC000992
• Gene Symbol: CSRP2
• Gene ID (NCBI): 1466
• RRID: AB_2087738
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Sci Rep; Proteotoxicity in cardiac amyloidosis: amyloidogenic light chains affect the levels of intracellular proteins in human heart cells.; Authors - Esther Imperlini
rat	WB,IF	Toxicol Appl Pharmacol; Cysteine and glycine rich protein 2 exacerbates vascular fibrosis in pulmonary hypertension through the nuclear translocation of yes-associated protein and transcriptional coactivator with PDZ-binding motif; Authors - Xinghe Chen ; KD Validated
human	WB,IHC	J Med Dent Sci; TDP-43 in the skin of amyotrophic lateral sclerosis patients.; Authors - Keisuke Abe
mouse	WB	Cell Biochem Funct; Cysteine and glycine-rich protein 2 retards platelet-derived growth factor-BB-evoked phenotypic transition of airway smooth muscle cells by decreasing YAP/TAZ activity; Authors - Huiyuan Wang ; KD Validated
human,mouse	WB,IHC	Toxicol Appl Pharmacol; Cysteine and glycine-rich protein 2 is crucial for maintaining the malignant phenotypes of gliomas through its action on Notch signalling cascade; Authors - Lingtong Liu