CSRP1 Polyclonal antibody

CSRP1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13432-1-AP

Synonyms

Cysteine rich protein 1, Cysteine and glycine-rich protein 1, CYRP, CSRP, CRP1

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Validation Data Gallery

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  WB analysis of human stomach using 13432-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 13432-1-AP (CSRP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse stomach using 13432-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 13432-1-AP (CSRP1 antibody) at dilution of 1:300.

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  IHC staining of human normal colon using 13432-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13432-1-AP (CSRP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human normal colon using 13432-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13432-1-AP (CSRP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 13432-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13432-1-AP (CSRP1 Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung cancer using 13432-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13432-1-AP (CSRP1 Antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: human stomach tissue
• Positive IP detected in: mouse stomach tissue
• Positive IHC detected in: human normal colon, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

13432-1-AP targets CSRP1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CSRP1 fusion protein Ag4236
• Full Name: cysteine and glycine-rich protein 1
• Calculated Molecular Weight: 193 aa, 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC032493
• Gene Symbol: CSRP1
• Gene ID (NCBI): 1465
• RRID: AB_2230180
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB	Mol Med Rep; Inflammatory and oxidative stress‑associated factors in chronic intermittent hypoxia in Chinese patients, rats, lymphocytes and endotheliocytes.; Authors - Hui Ren