CSPG4 Polyclonal antibody

CSPG4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31623-1-AP

Synonyms

NG2, MSK16, Melanoma-associated chondroitin sulfate proteoglycan, MCSPG, MCSP

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Validation Data Gallery

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  WB analysis using 31623-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31623-1-AP (CSPG4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31623-1-AP targets CSPG4 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: chondroitin sulfate proteoglycan 4
• Calculated Molecular Weight: 251kd
• Observed Molecular Weight: 250-300 kDa
• GenBank Accession Number: NM_001897.5
• Gene Symbol: CSPG4
• Gene ID (NCBI): 1464
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CSPG4 is a proteoglycan that plays a role in cell proliferation and migration, stimulates endothelial cell motility during microvascular morphogenesis and may also inhibit neurite outgrowth and growth cone collapse during axon regeneration. CSPG4 is expressed on more than 90% of human melanoma tissues and cultured cells. It is a target of specific diagnosis and immunotherapy with antiidiotypic antibodies due, in part, to its roles in growth control, adhesion, cell-substratum interactions, and cell-cell contacts (PMID: 10587647; 11278606).