CSNK2A2 Polyclonal antibody

CSNK2A2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 10606-1-AP

Synonyms

EC:2.7.11.1, CSNK2A1, CSNK2A 2

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Validation Data Gallery

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  WB analysis using 10606-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10606-1-AP (CSNK2A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 10606-1-AP

  IP result of anti-CSNK2A2 (IP:10606-1-AP, 4ug; Detection:10606-1-AP 1:8000) with Jurkat cells lysate 1400 ug.

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  IF Staining of HepG2 using 10606-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CSNK2A2 antibody (10606-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, Jurkat cells
• Positive IP detected in: Jurkat cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Product Information

10606-1-AP targets CSNK2A2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CSNK2A2 fusion protein Ag0877
• Full Name: casein kinase 2, alpha prime polypeptide
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: BC008812
• Gene Symbol: CSNK2A2
• Gene ID (NCBI): 1459
• RRID: AB_2292447
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CSNK2A2(Casein kinase II subunit alpha') is also named as CK2A3, CK2A2, CSNK2A1, CK2 and belongs to the protein kinase superfamily. It can function as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage during mitosis. It also can protect against ionizing radiation-induced apoptosis partly by phosphorylating and activating SIRT1. This protein has molecular weights between 35 kDa and 44 kDa(PMID:8622692).

Publications

Species	Application	Title
mouse	WB,IHC	Acta Neuropathol Commun; CK2 alpha prime and alpha-synuclein pathogenic functional interaction mediates synaptic dysregulation in huntington's disease.; Authors - Dahyun Yu
mouse	WB	Biochem Biophys Res Commun; β-catenin stimulates Tcf7l1 degradation through recruitment of casein kinase 2 in mouse embryonic stem cells.; Authors - Yan Zhang
mouse	WB	Cell Rep; Tumor suppressor p53 regulates heat shock factor 1 protein degradation in Huntington's disease; Authors - Rachel H Mansky ; KD Validated
human	WB,IP	Cell Genom; NRDE2 deficiency impairs homologous recombination repair and sensitizes hepatocellular carcinoma to PARP inhibitors; Authors - Yahui Wang ; KD Validated
human	WB	Int Immunopharmacol; A quinolinyl resveratrol derivative alleviates acute ischemic stroke injury by promoting mitophagy for neuroprotection via targeting CK2α'; Authors - Libin Xu