CSNK2A1 Monoclonal antibody
CSNK2A1 Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, rabbit
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1D5E8
Cat no : 68200-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | LNCaP cells, rabbit heart tissue, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, MOLT-4 cells, K-562 cells, HL-60 cells |
Positive IF/ICC detected in | A431 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:750-1:3000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
68200-1-Ig targets CSNK2A1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rabbit samples.
Tested Reactivity | human, rabbit |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | CSNK2A1 fusion protein Ag29062 |
Full Name | casein kinase 2, alpha 1 polypeptide |
Calculated Molecular Weight | 45 kDa |
Observed Molecular Weight | 40-45 kDa |
GenBank Accession Number | BC011668 |
Gene Symbol | CSNK2A1 |
Gene ID (NCBI) | 1457 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CSNK2A1(Casein kinase II subunit alpha) is also named as CK2A1 and belongs to the CK2 subfamily. The CSNK2A1 gene contains 8 exons whose sequences comprise bases 102 to 824 of the coding region of the human casein kinase II alpha subunit. Casein kinase II is a ubiquitous protein-serine/threonine kinase in eukaryotic cells that catalyzes the phosphorylation of many protein substrates and has been regarded as a second-messenger-independent protein kinase. This enzyme is composed of an α subunit (37 to 44kDa) and a β subunit (24 to 28 kDa) and exists as an α2β2 tetramer (PMID:1448100). Casein kinase 2 has been found to be increased at protein level and up-regulated at the level of enzyme activity in the majority of cancers. However, elevated levels of casein kinase 2 are present in certain normal organs such as brain and testes.
Protocols
Product Specific Protocols | |
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WB protocol for CSNK2A1 antibody 68200-1-Ig | Download protocol |
IF protocol for CSNK2A1 antibody 68200-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |