CSNK2A1 Monoclonal antibody

CSNK2A1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rabbit

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1D5E8

Cat no : 68200-1-Ig

Synonyms

CKII, CK2α, CK2A1, CK2a, CK II alpha

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Validation Data Gallery

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  WB analysis using 68200-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68200-1-Ig (CSNK2A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of rabbit heart using 68200-1-Ig

  rabbit heart tissue were subjected to SDS PAGE followed by western blot with 68200-1-Ig (CSNK2A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 68200-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CSNK2A1 antibody (68200-1-Ig, Clone: 1D5E8 ) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: LNCaP cells, rabbit heart tissue, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, MOLT-4 cells, K-562 cells, HL-60 cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:750-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IP: See 1 publications below

Product Information

68200-1-Ig targets CSNK2A1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, rabbit samples.

• Tested Reactivity: human, rabbit
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CSNK2A1 fusion protein Ag29062
• Full Name: casein kinase 2, alpha 1 polypeptide
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC011668
• Gene Symbol: CSNK2A1
• Gene ID (NCBI): 1457
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CSNK2A1(Casein kinase II subunit alpha) is also named as CK2A1 and belongs to the CK2 subfamily. The CSNK2A1 gene contains 8 exons whose sequences comprise bases 102 to 824 of the coding region of the human casein kinase II alpha subunit. Casein kinase II is a ubiquitous protein-serine/threonine kinase in eukaryotic cells that catalyzes the phosphorylation of many protein substrates and has been regarded as a second-messenger-independent protein kinase. This enzyme is composed of an α subunit (37 to 44kDa) and a β subunit (24 to 28 kDa) and exists as an α2β2 tetramer (PMID:1448100). Casein kinase 2 has been found to be increased at protein level and up-regulated at the level of enzyme activity in the majority of cancers. However, elevated levels of casein kinase 2 are present in certain normal organs such as brain and testes.

Publications

Species	Application	Title
human	WB,IP	F1000Res; A guide to selecting high-performing antibodies for CSNK2A1 (UniProt ID: P68400) for use in western blot, immunoprecipitation and immunofluorescence; Authors - Riham Ayoubi ; KO Validated