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GM-CSF Polyclonal antibody

GM-CSF Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human and More (3)





Cat no : 17762-1-AP


Colony stimulating factor, CSF, CSF2, GM CSF, GMCSF, GM-CSF, Molgramostin, Sargramostim

Tested Applications

Positive WB detected inNK-92 cells
Positive IHC detected inhuman tonsillitis tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17762-1-AP targets GM-CSF in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GM-CSF fusion protein Ag12142
Full Name colony stimulating factor 2 (granulocyte-macrophage)
Calculated Molecular Weight 144 aa, 16 kDa
Observed Molecular Weight15kDa,18 kDa ,22 kDa
GenBank Accession NumberBC108724
Gene Symbol GM-CSF
Gene ID (NCBI) 1437
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts. GM-CSF is a hematopoietic growth factor that stimulates the development of early erythroid megakaryocytic and eosinophilic progenitor cells (PMID 2990035). GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes (PMID 3021817, 2984574, 6390681).


Product Specific Protocols
WB protocol for GM-CSF antibody 17762-1-APDownload protocol
IHC protocol for GM-CSF antibody 17762-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Hematol Oncol

FFAR2 expressing myeloid-derived suppressor cells drive cancer immunoevasion

Authors - Zeda Zhao

J Exp Med

Tumoral EHF predicts the efficacy of anti-PD1 therapy in pancreatic ductal adenocarcinoma.

Authors - Jing Liu

J Am Soc Nephrol

Tubular GM-CSF Promotes Late MCP-1/CCR2-Mediated Fibrosis and Inflammation after Ischemia/Reperfusion Injury.

Authors - Leyuan Xu

Acta Pharmacol Sin

Macrophages promote the transition from myocardial ischemia reperfusion injury to cardiac fibrosis in mice through GMCSF/CCL2/CCR2 and phenotype switching

Authors - Shi-Chun Shen


CMAHP promotes metastasis by reducing ubiquitination of Snail and inducing angiogenesis via GM-CSF overexpression in gastric cancer.

Authors - Hsiang-Wei Huang

Int J Mol Sci

Regulation of Th17/Treg Balance by 27-Hydroxycholesterol and 24S-Hydroxycholesterol Correlates with Learning and Memory Ability in Mice.

Authors - Tao Wang