CSDC2 Recombinant antibody

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  WB analysis using 83886-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83886-5-RR (CSDC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83886-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83886-5-RR against Human CSDC2 were performed. The affinity constant is 1.49 nM.

CSDC2 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 83886-5-RR

Clone No.241031C8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

RNA-binding protein PIPPin, RNA binding protein PIPPin, PIPPIN, Cold shock domain-containing protein C2, 241031C8

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Tested Applications

• Positive WB detected in: HeLa cells, U2OS cells, U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83886-5-RR targets CSDC2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CSDC2 fusion protein Ag16216
• Full Name: cold shock domain containing C2, RNA binding
• Calculated Molecular Weight: 153 aa, 17 kDa
• Observed Molecular Weight: 20 kDa, 30 kDa
• GenBank Accession Number: BC067113
• Gene Symbol: CSDC2
• Gene ID (NCBI): 27254
• RRID: AB_3671468
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9Y534
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIPPIN, also known as CSDC2 (Cold shock domain-containing protein C2) is an RNA‐binding protein (RBP), highly enriched in the rat brain, specifically enriched in some pyramidal neurons of the cerebral cortex and in the Purkinje cells of the cerebellum (PMID: 10446180). PIPPIN has the potential to undergo different posttranslational modifications and might be a good candidate to regulate the synthesis of specific proteins in response to extracellular stimuli. Nuclear CSDC2 is connected to proliferation and cytoplasmic CSDC2 to terminal differentiation in the decidua and that CSDC2 could regulate differentiation during decidua development (PMID: 30078185, PMID: 17053029).