CSDA Polyclonal antibody

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  WB analysis using 31704-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 31704-1-AP (CSDA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of KYSE-30 using 31704-1-AP

  WB result of CSDA antibody (31704-1-AP; 1:1000; room temperature for 1.5 hours) with wild-type and CSDA knockout KYSE-30 cells.

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  IF Staining of U2OS using 31704-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CSDA antibody (31704-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

• Featured Product
• KD/KO Validated

CSDA Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 31704-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Y-box-binding protein 3, Single-strand DNA-binding protein NF-GMB, DNA-binding protein A, DBPA, Cold shock domain-containing protein A

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Tested Applications

• Positive WB detected in: mouse heart tissue, KYSE-30 cells, mouse liver tissue, mouse skeletal muscle tissue
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31704-1-AP targets CSDA in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CSDA fusion protein Ag36519
• Full Name: cold shock domain protein A
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 60-68 kDa
• GenBank Accession Number: BC008801
• Gene Symbol: CSDA
• Gene ID (NCBI): 8531
• RRID: AB_3670088
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P16989
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CSDA (Cold shock domain protein A), also known as DBPA (DNA-binding protein A), YBX3 (Y-box protein 3), is a DNA-binding protein that represses angiogenesis and lymphangiogenesis by directly binding to hypoxia response element (HRE) and serum response element (SRE). CSDA is ubiquitous expressed in human skeletal muscle, heart, and decidual cells, which is thought to a multifunctional epithelial-specific protein involved in gene transcription, DNA repair, and interaction with other proteins (PMID: 33816248). CSDA is a nucleic-acid-binding protein that directly regulates gene expression by using different mechanisms, and acts as a translational repressor (e.g., MYH9 and SPTBN1) and positive regulator of mRNA stability (PMID: 31189097). Western blot analysis detected CDSA at a range of 50~70 kDa due to the anomalous mobility of Y-box proteins (PMID: 38255791).