CSDA Polyclonal antibody

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  WB analysis using 27785-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27785-1-AP (CSDA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of KYSE-30 using 27785-1-AP

  WB result of CSDA antibody (27785-1-AP; 1:1000; room temperature for 1.5 hours) with wild-type and CSDA knockout KYSE-30 cells.

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  IHC staining of mouse heart using 27785-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27785-1-AP (CSDA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 27785-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27785-1-AP (CSDA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CSDA Polyclonal Antibody for WB, IHC, ELISA

Cat No. 27785-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Y-box-binding protein 3, Single-strand DNA-binding protein NF-GMB, DNA-binding protein A, DBPA, Cold shock domain-containing protein A

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Tested Applications

• Positive WB detected in: K-562 cells, Y79 cells, mouse liver tissue
• Positive IHC detected in: mouse heart tissue, mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

27785-1-AP targets CSDA in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CSDA fusion protein Ag26839
• Full Name: cold shock domain protein A
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 60-68 kDa
• GenBank Accession Number: BC008801
• Gene Symbol: CSDA
• Gene ID (NCBI): 8531
• RRID: AB_3085996
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P16989
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CSDA (Cold shock domain protein A), also known as DBPA (DNA-binding protein A), YBX3 (Y-box protein 3), is a DNA-binding protein that represses angiogenesis and lymphangiogenesis by directly binding to hypoxia response element (HRE) and serum response element (SRE). CSDA is ubiquitous expressed in human skeletal muscle, heart, and decidual cells, which is thought to a multifunctional epithelial-specific protein involved in gene transcription, DNA repair, and interaction with other proteins (PMID: 33816248). CSDA is a nucleic-acid-binding protein that directly regulates gene expression by using different mechanisms, and acts as a translational repressor (e.g., MYH9 and SPTBN1) and positive regulator of mRNA stability (PMID: 31189097). Western blot analysis detected CDSA at a range of 50~70 kDa due to the anomalous mobility of Y-box proteins (PMID: 38255791).

Publications

Species	Application	Title
mouse	WB	Mol Metab; RNA-binding protein YBX3 promotes PPARγ-SLC3A2 mediated BCAA metabolism fueling brown adipogenesis and thermogenesis; Authors - Lin-Yun Chen