CRYGS Polyclonal antibody

CRYGS Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 16542-1-AP

Synonyms

Beta crystallin S, CRYG8, CRYGS, crystallin, gamma S, Gamma crystallin S, Gamma S crystallin, GRYG8

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Validation Data Gallery

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  WB analysis using 16542-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16542-1-AP (CRYGS antibody) at dilution of 1:80000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: rat eye tissue

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16542-1-AP targets CRYGS in WB, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CRYGS fusion protein Ag9868
• Full Name: crystallin, gamma S
• Calculated Molecular Weight: 178 aa, 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC070241
• Gene Symbol: CRYGS
• Gene ID (NCBI): 1427
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CRYGS (Gamma-S crystallin), also called CRYG8, belongs to the gamma-crystallins (PMID: 7733876; 23199295). Three major classes of crystallins, alpha, beta, and gamma, are common to the eye lens throughout all vertebrates. The beta and gamma classes are made up of homologous proteins and constitute the beta/gamma crystallin superfamily. CRYGS is expressed late but abundantly in the ocular lens (PMID: 1445197). It is also expressed outside the lens, particularly in the mature retina and cornea. In contrast to the beta-crystallins which associate in various combinations to form low or high molecular weight aggregates, CRYGS is, like the other gamma-crystallins, a monomeric protein (PMID: 16141006).