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Cryptochrome 1 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 0.781-100 ng/mL

Sandwich ELISA: 3.13-200 ng/mL

Cat No : MP00603-3

Print datasheet

Synonyms

CRY1, Cryptochrome 1, PHLL1

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00603-3, Human Cryptochrome 1 Recombinant Matched Antibody Pair - PBS only. 83602-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag4270. 83602-1-PBS was HRP conjugated as the detection antibody. Range: 3.13-200 ng/mL.

    Sandwich ELISA standard curve of MP00603-3

    Sandwich ELISA standard curve of MP00603-3, Human Cryptochrome 1 Recombinant Matched Antibody Pair - PBS only. 83602-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag4270. 83602-1-PBS was HRP conjugated as the detection antibody. Range: 3.13-200 ng/mL.

  • The mean Cryptochrome 1 concentration was determined to be 21.76 ng/mL in HeLa cell extract based on a 1.20 mg/mL extract load.

    Sample test of MP00603-3

    The mean Cryptochrome 1 concentration was determined to be 21.76 ng/mL in HeLa cell extract based on a 1.20 mg/mL extract load.

  • Cytometric bead array standard curve of MP00603-3, CRY1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83602-3-PBS. Detection antibody: 83602-1-PBS. Standard: Ag4270. Range: 0.781-100 ng/mL.

    Cytometric bead array standard curve of MP00603-3

    Cytometric bead array standard curve of MP00603-3, CRY1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83602-3-PBS. Detection antibody: 83602-1-PBS. Standard: Ag4270. Range: 0.781-100 ng/mL.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00603-3 targets Cryptochrome 1 in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 0.781-100 ng/mL (Cytometric Bead Array); 3.13-200 ng/mL (Sandwich ELISA);

Capture antibody: Cryptochrome 1 Recombinant antibody, PBS Only (Capture/Detector) 83602-3-PBS (240520B4). 100 μg. Concentration 1 mgl/ml.

Detection antibody: Cryptochrome 1 Recombinant antibody, PBS Only (Detector) 83602-1-PBS (240520A12). 100 μg. Concentration 1 mgl/ml.

Alternative Cryptochrome 1 matched antibody pairs: MP00603-1, MP00603-2

Matched pair protein standard: Ag4270

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83602-3-PBS
Tested Reactivity human
Immunogen Cryptochrome 1 fusion protein Ag4270
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cryptochrome 1 (photolyase-like)
Synonyms CRY1, Cryptochrome-1, CRY 1, 240520B4
GenBank accession numberBC030519
Gene symbol CRY1
UniprotQ16526
Gene ID (NCBI) 1407
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 83602-1-PBS
Tested Reactivity human
Immunogen Cryptochrome 1 fusion protein Ag4270
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cryptochrome 1 (photolyase-like)
Synonyms CRY1, Cryptochrome-1, CRY 1, 240520A12
GenBank accession numberBC030519
Gene symbol CRY1
UniprotQ16526
Gene ID (NCBI) 1407
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.