Cryptochrome 1 Recombinant antibody

• 

  WB analysis using 83602-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83602-5-RR (CRY1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 83602-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83602-5-RR against Human Cryptochrome 1 were performed. The affinity constant is below 1 pM.

Cryptochrome 1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 83602-5-RR

Clone No.240520B12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CRY1, Cryptochrome-1, CRY 1, 240520B12

---

Tested Applications

• Positive WB detected in: HeLa cells, Y79 cells, HCT 116 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83602-5-RR targets Cryptochrome 1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Cryptochrome 1 fusion protein Ag4270
• Full Name: cryptochrome 1 (photolyase-like)
• Calculated Molecular Weight: 586 aa, 66 kDa
• Observed Molecular Weight: 60-66 kDa
• GenBank Accession Number: BC030519
• Gene Symbol: CRY1
• Gene ID (NCBI): 1407
• RRID: AB_3671216
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q16526
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cryptochrome circadian clock 1 (CRY1) is a flavin adenine dinucleotide-binding protein with MW of 66 kDa. CRY1 is a key component of the circadian core oscillator complex, which regulates the circadian clock. CRY1 predominantly displays evening-time expression and serves as a strong repressor of morning-time elements (E box/E-prime box) when bound to the BMAL1 (ARNTL; 602550)/CLOCK (601851) complex (PubMed: 21236481).