CRX Polyclonal antibody

CRX Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 12047-1-AP

Synonyms

cone rod homeobox, Cone rod homeobox protein, CORD2, CRD, CRX, diencephalon/mesencephalon homeobox 1, DMBX1, LCA7, OTX3

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Validation Data Gallery

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  WB analysis of Y79 using 12047-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12047-1-AP (CRX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 12047-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12047-1-AP (CRX antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of Y79 using 12047-1-AP

  IP result of anti-CRX (IP:12047-1-AP, 4ug; Detection:12047-1-AP 1:500) with Y79 cells lysate 1920ug.

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  IHC staining of mouse testis using 12047-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12047-1-AP (CRX antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse eye using 12047-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 12047-1-AP (CRX antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Y79 cells
• Positive IP detected in: Y79 cells
• Positive IHC detected in: mouse eye tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

12047-1-AP targets CRX in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CRX fusion protein Ag2677
• Full Name: cone-rod homeobox
• Calculated Molecular Weight: 299 aa, 32 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC016664
• Gene Symbol: CRX
• Gene ID (NCBI): 1406
• RRID: AB_2292128
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cone-rod homeobox (Crx) is a homeodomain transcription factor and member of the Otx family, which has been thought to play a critical role in determining and maintaining the phenotype of both pinealocytes and retinal photoreceptors [PMID:17467693]. In the mammalian retina, Crx plays an essential role in the normal development and maintenance of cones and rods and regulates expression of the network of genes that characterize the retina [PMID:17653270]. Elimination of Crx by disruption of the homeobox domain results in loss of the image-forming visual system but not the non-image-forming visual system controlling circadian rhythms [PMID:20438719].

Publications

Species	Application	Title
human	WB, CoIP	J Neurosci; The transcription factor GTF2IRD1 regulates the topology and function of photoreceptors by modulating photoreceptor gene expression across the retina.; Authors - Tomohiro Masuda
rat	IF	iScience; Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration; Authors - Haibin Tian
mouse	IHC	Dev Dyn; Reduced mTORC1-signaling in progenitor cells leads to retinal lamination deficits; Authors - Christoffer Nord
rat	IF	Neural Regen Res; Müller cells are activated in response to retinal outer nuclear layer degeneration in rats subjected to simulated weightlessness conditions; Authors - Yuxue Mu