C-Reactive Protein/CRP Polyclonal antibody

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  WB analysis using 24175-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24175-1-AP (C-Reactive Protein/CRP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of serum from mouse injected with bacteria using 24175-1-AP

  Serum from mouse injected with bacteria were subjected to SDS PAGE followed by western blot with 24175-1-AP (C-Reactive Protein/CRP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 24175-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 24175-1-AP (C-Reactive Protein/CRP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 24175-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24175-1-AP (C-Reactive Protein/CRP Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 24175-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24175-1-AP (C-Reactive Protein/CRP Antibody) at dilution of 1:200 (under 40x lens).

C-Reactive Protein/CRP Polyclonal Antibody for WB, IHC, ELISA

Cat No. 24175-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CRP, PTX1, C-reactive protein(1-205), C-Reactive Protein, C reactive protein

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Tested Applications

• Positive WB detected in: rat plasma, serum from mouse injected with bacteria, human plasma
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

24175-1-AP targets C-Reactive Protein/CRP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C-Reactive Protein/CRP fusion protein Ag21466
• Full Name: C-reactive protein, pentraxin-related
• Calculated Molecular Weight: 224 aa, 25 kDa
• Observed Molecular Weight: 25-28 kDa
• GenBank Accession Number: BC125135
• Gene Symbol: CRP
• Gene ID (NCBI): 1401
• RRID: AB_2879447
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: P02741
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C-reactive protein (CRP) is an acute-phase serum protein synthesized by the liver in response to interleukin-6 (IL-6) during inflammation. The name of CRP derives from its ability to react with the C polysaccharide of Streptococcus pneumoniae. CRP is an annular, pentameric protein that belongs to the pentraxin family of proteins. CRP displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It is used mainly as a marker of inflammation.

Publications

Species	Application	Title
human, mouse	WB	J Proteome Res; Identification of Organ-Enriched Protein Biomarkers of Acute Liver Injury by Targeted Quantitative Proteomics of Blood in Acetaminophen- and Carbon-Tetrachloride-Treated Mouse Models and Acetaminophen Overdose Patients.; Authors - Shizhen Qin
human	WB	J Proteome Res; Measurement of Organ-Specific and Acute-Phase Blood Protein Levels in Early Lyme Disease.; Authors - Yong Zhou
human	WB	Biomed Mater; Reversing cytotoxicity of uric acid by supramolecular encapsulation with acyclic cucurbit[n]uril.; Authors - Lingguang Kong
mouse	IHC	Biochem Biophys Res Commun; In vitro and in vivo study of andrographolide nanoparticles for the treatment of Mycoplasma pneumoniae pneumonia; Authors - Liling Wang
		Exp Ther Med; TLR4 gene in the regulation of periodontitis and its molecular mechanism.; Authors - Weijuan Qi
rat	WB	ACS Omega; TMT Labeling Reveals the Effects of Exercises on the Proteomic Characteristics of the Subcutaneous Adipose Tissue of Growing High-Fat-Diet-Fed Rats; Authors - Ge Song