CRM1 Polyclonal antibody

CRM1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (2)

Cat no : 27917-1-AP

Synonyms

CRM1, Exp1, Exportin 1, XPO1

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Validation Data Gallery

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  WB analysis using 27917-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27917-1-AP (CRM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 27917-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 27917-1-AP (CRM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27917-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27917-1-AP (CRM1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 27917-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27917-1-AP (CRM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 27917-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27917-1-AP (CRM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, C6 cells, HeLa cells, MCF-7 cells, NIH/3T3 cells, K-562 cells, HT-1080 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

27917-1-AP targets CRM1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CRM1 fusion protein Ag27207
• Full Name: exportin 1 (CRM1 homolog, yeast)
• Calculated Molecular Weight: 1071 aa, 123 kDa
• Observed Molecular Weight: 123 kDa
• GenBank Accession Number: BC032847
• Gene Symbol: CRM1
• Gene ID (NCBI): 7514
• RRID: AB_2881009
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CRM1 (chromosome region maintenance 1, also known as exportin-1 or XPO1), a member of the karyopherin β family of transport receptors, is an important nuclear export receptor recognizing proteins bearing a leucine-rich nuclear export signal (PMID: 9323133). CRM1 is the major receptor for the export of proteins out of the nucleus and is also required for transport of many RNAs (PMID: 17317185). In addition to nuclear export, CRM1 also plays a role in centrosome duplication and spindle assembly (PMID: 22773955). Elevated CRM1 expression has been reported in several cancers, which implicates CRM1 as a potential anti-cancer drug target (PMID: 22677130; 21683812 ).

Publications

Species	Application	Title
	WB,CoIP	J Immunol; YTHDC1/CRM1 Facilitates m6A-Modified circRNA388 Nuclear Export to Induce Coelomocyte Autophagy via the miR-2008/ULK Axis in Apostichopus japonicus; Authors - Jiqing Liu
human	IF	J Cell Commun Signal; CD47 interactions with exportin-1 limit the targeting of m 7 G-modified RNAs to extracellular vesicles; Authors - Sukhbir Kaur
mouse	IHC	J Cancer Res Clin Oncol; Selinexor demonstrates anti-tumor efficacy in paired patient-derived xenograft models and hydrogel-embedded histoculture drug sensitivity test of penile cancer; Authors - Yuanqiao He
human	WB	Mol Cell; EGFR promotes ALKBH5 nuclear retention to attenuate N6-methyladenosine and protect against ferroptosis in glioblastoma; Authors - Deguan Lv
mouse	WB	Mol Cell; A GAPDH serotonylation system couples CD8+ T cell glycolytic metabolism to antitumor immunity; Authors - Xu Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xingyuan (Verified Customer) (07-17-2023)

The band quality was pretty good

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HEK293T

CRM1 Antibody Western Blot validation (1:5000 dilution) in HEK293T (Cat no:27917-1-AP)

FH

Wei (Verified Customer) (03-12-2020)

strong, clear band for mouse heart lysates

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart