CRK Polyclonal antibody

CRK Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 16685-1-AP

Synonyms

Adapter molecule crk, CRK, CRKII, p38, Proto oncogene c Crk

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 16685-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16685-1-AP (CRK Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 16685-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16685-1-AP (CRK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 16685-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16685-1-AP (CRK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 16685-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16685-1-AP (CRK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human urothelial carcinoma using 16685-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 16685-1-AP (CRK antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 16685-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 16685-1-AP (CRK antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 16685-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 16685-1-AP (CRK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of PC-3 using 16685-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 16685-1-AP (CRK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, A549 cells, PC-3 cells
• Positive IHC detected in: human urothelial carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16685-1-AP targets CRK in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CRK fusion protein Ag10016
• Full Name: v-crk sarcoma virus CT10 oncogene homolog (avian)
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC008506
• Gene Symbol: CRK
• Gene ID (NCBI): 1398
• RRID: AB_2084893
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.