CRISP3 Polyclonal antibody

• Featured Product
• KD/KO Validated

CRISP3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 14847-1-AP

Synonyms

Aeg2, CRISP 3, CRISP3, CRS3, SGP28

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Validation Data Gallery

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  WB analysis using 14847-1-AP

  human saliva samples were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 14847-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 14847-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 14847-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 14847-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 14847-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 14847-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14847-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 14847-1-AP

  IP result of anti-CRISP3 (IP:14847-1-AP, 4ug; Detection:14847-1-AP 1:300) with human placenta tissue lysate 1520ug.

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  IHC staining of human prostate cancer using 14847-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14847-1-AP (CRISP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 14847-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14847-1-AP (CRISP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 14847-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14847-1-AP (CRISP3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 14847-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14847-1-AP (CRISP3 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human saliva, human placenta tissue, Jurkat cells, mouse colon tissue, mouse ovary tissue, mouse thymus tissue
• Positive IP detected in: human placenta tissue
• Positive IHC detected in: human prostate cancer tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 3 publications below

Product Information

14847-1-AP targets CRISP3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: cysteine-rich secretory protein 3
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: NM_006061
• Gene Symbol: CRISP3
• Gene ID (NCBI): 10321
• RRID: AB_1607510
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CRISP3, also known as SGP28, is a glycoprotein that belongs to a family of cysteine-rich secretory proteins (CRISPs). CRISP3 was originally discovered in human neutrophils, but transcripts are widely distributed in exocrine glands (salivary glands, pancreas, and prostate) and also found at lower levels in epididymis, ovary, thymus, and colon. CRISP3 is a potential biomarker for prostate cancer. This antibody can recognize both CRISP2 and CRISP3.

Publications

Species	Application	Title
human	IHC	J Natl Cancer Inst; Progesterone Receptor Isoform Ratio: A Breast Cancer Prognostic and Predictive Factor for Antiprogestin Responsiveness.; Authors - Paola A Rojas
human	IHC	J Hematol Oncol; Cysteine- rich secretory protein 3 (CRISP3), ERG and PTEN define a molecular subtype of prostate cancer with implication to patients' prognosis.; Authors - Samir Al Bashir
human	WB,IHC	Int J Biol Sci; Circular RNA hsa_circ_0003823 promotes the Tumor Progression, Metastasis and Apatinib Resistance of Esophageal Squamous Cell Carcinoma by miR-607/CRISP3 Axis; Authors - Yu-Ming Wang ; KD Validated
human	WB	J Dent Res; Proteome Analysis of Temporomandibular Joint with Disc Displacement; Authors - X Liu