CRH/CRF Polyclonal antibody

CRH/CRF Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 26848-1-AP

Synonyms

CRH, CRF, Corticotropin-releasing hormone, Corticotropin-releasing factor, Corticotropin releasing factor

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Validation Data Gallery

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  WB analysis using 26848-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26848-1-AP (CRH/CRF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 26848-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26848-1-AP (CRH/CRF antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 26848-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26848-1-AP (CRH/CRF antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat brain using 26848-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CRH/CRF antibody (26848-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: rat brain tissue, mouse brain tissue
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26848-1-AP targets CRH/CRF in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CRH/CRF fusion protein Ag25226
• Full Name: CRH/CRF
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC011031
• Gene Symbol: CRH
• Gene ID (NCBI): 1392
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CRH, also called CRF or corticoliberin, is a peptide hormone and neurotransmitter involved in the stress response. Marked reduction in this protein has been observed in association with Alzheimer disease. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery.