CREST Polyclonal antibody

• Featured Product
• KD/KO Validated

CREST Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

10

Reviews (1)

Cat no : 12439-1-AP

Synonyms

SS18L1, SS18-like protein 1, SS18 like protein 1, LP2261, KIAA0693

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Validation Data Gallery

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  WB analysis using 12439-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12439-1-AP (CREST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12439-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12439-1-AP (CREST antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12439-1-AP

  WB result of CREST antibody (12439-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CREST transfected HeLa cells.

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  WB analysis of human heart using 12439-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12439-1-AP (CREST antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 12439-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12439-1-AP (CREST antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12439-1-AP

  IP result of anti-CREST (IP:12439-1-AP, 4ug; Detection:12439-1-AP 1:300) with HeLa cells lysate 3000 ug.

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  IP experiment of mouse brain using 12439-1-AP

  IP result of anti-CREST (IP:12439-1-AP, 3ug; Detection:12439-1-AP 1:1000) with mouse brain tissue lysate 6000ug.

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  IHC staining of human breast cancer using 12439-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 12439-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 12439-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human breast cancer using 12439-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 12439-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12439-1-AP (CREST antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 12439-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12439-1-AP (CREST antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 12439-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 12439-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 12439-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 12439-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 12439-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of mouse testis using 12439-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 12439-1-AP (CREST antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse testis using 12439-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 12439-1-AP (CREST antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 12439-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12439-1-AP (CREST antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 12439-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CREST antibody (12439-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 12439-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12439-1-AP (CREST antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: K-562 cells, COLO 320 cells, HeLa cells, human heart tissue, mouse brain tissue, rat brain tissue
• Positive IP detected in: HeLa cells, mouse brain tissue
• Positive IHC detected in: human breast cancer tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse testis tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 6 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Product Information

12439-1-AP targets CREST in WB, IHC, IF/ICC, IF-P, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, drosophila
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CREST fusion protein Ag3119
• Full Name: synovial sarcoma translocation gene on chromosome 18-like 1
• Calculated Molecular Weight: 396 aa, 43 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC034494
• Gene Symbol: CREST
• Gene ID (NCBI): 26039
• RRID: AB_2302517
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CREST, also named as SS18-like 1(SS18L1) is a transcriptional activator that is required for calcium-dependent dendritic growth and branching in cortical neurons. It's also a nuclear protein interacts with CREB-binding protein and expressed in the developing brain. It helps regulate neuronal morphogenesis in calcuim-dependent manner. The N-terminal domain of SS18L1 is required for suppressing transactivation in the basal state, while the C-terminal domain is required for calcium-induced transactivation. It's part of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. This complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. The calculated molecular weight of CREST is about 43 kDa, but the modified of CREST protein is 55 kDa (PMID: 25888396). CREST exists some isoforms with calulated MV 43, 40, 33 and 29 kDa.

Publications

Species	Application	Title
human, mouse	IHC,CoIP	Nat Neurosci; Exome sequencing to identify de novo mutations in sporadic ALS trios.; Authors - Chesi Alessandra A
mouse	IF	J Neurosci; Kinetic Analysis of npBAF to nBAF Switching Reveals Exchange of SS18 with CREST and Integration with Neural Developmental Pathways.; Authors - Staahl Brett T BT
human	ChIP	Cancers (Basel); Global Chromatin Changes Resulting from Single-Gene Inactivation-The Role of SMARCB1 in Malignant Rhabdoid Tumor.; Authors - Colin Kenny
rat	WB,IF	J Neurosci; CREST in the Nucleus Accumbens Core Regulates Cocaine Conditioned Place Preference, Cocaine-Seeking Behavior, and Synaptic Plasticity.; Authors - Yasaman Alaghband
Drosophila	WB	PLoS Genet; Calcium-responsive transactivator (CREST) toxicity is rescued by loss of PBP1/ATXN2 function in a novel yeast proteinopathy model and in transgenic flies.; Authors - Sangeun Park
mouse	IF	Hum Mol Genet; Dysregulation of chromatin remodelling complexes in amyotrophic lateral sclerosis.; Authors - Michael Tibshirani

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aktan (Verified Customer) (12-24-2019)

The product is tested in prostate cancer cell lines. Used at the given dilution factor, in 5% BSA in PBST, o/n at 4C. Bands were detected using Licor system and Licor near IR secondary antibodies.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Prostate cancer cell lines

CREST Antibody Western Blot, validation (1:500 dilution) in Prostate cancer cell lines (Cat no:12439-1-AP)

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