CREB5 Polyclonal antibody

• Featured Product
• KD/KO Validated

CREB5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 14196-1-AP

Synonyms

CRE-BPa, CREBPA, CREB-5, CREB 5, cAMP-responsive element-binding protein 5

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Validation Data Gallery

• 

  WB analysis of HepG2 using 14196-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14196-1-AP (CREB5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14196-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14196-1-AP (CREB5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14196-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14196-1-AP (CREB5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 14196-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14196-1-AP (CREB5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, human brain tissue, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Product Information

14196-1-AP targets CREB5 in WB, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CREB5 fusion protein Ag5399
• Full Name: cAMP responsive element binding protein 5
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC059400
• Gene Symbol: CREB5
• Gene ID (NCBI): 9586
• RRID: AB_2083832
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

cyclic AMP responsive element binding protein 5 (CREB5), also known as CRE-BPA, a member of the cAMP response element binding protein family, is a transcription factor specifically expressed in superficial zone articular chondrocytes and is required for TGF-β and EGFR signaling. Knockdown of CREB5 decreased the c-jun and c-fos expression levels in HC-a cells (PMID: 38549717). Increased CREB5 expression is positively correlated with tumor cell invasion and a poor prognosis for cancer patients with epithelial ovarian cancer and hepatocellular carcinoma (PMID: 38418476).This protein specifically binds to CRE as a homodimer or a heterodimer with c-Jun or CRE-BP1, and functions as a CRE-dependent trans-activator. Alternatively spliced transcript variants encoding different isoforms have been identified (PMID: 33712729).

Publications

Species	Application	Title
human	WB	Int J Hyperthermia; Hyperthermia promotes exosome secretion by regulating Rab7b while increasing drug sensitivity in adriamycin-resistant breast cancer.; Authors - Di Xu
human	WB	J Ovarian Res; Role of BMI1 in epithelial ovarian cancer: investigated via the CRISPR/Cas9 system and RNA sequencing.; Authors - Qianying Zhao
human	WB	PLoS One; AT-hook DNA-binding motif-containing protein one knockdown downregulates EWS-FLI1 transcriptional activity in Ewing's sarcoma cells; Authors - Takao Kitagawa
human	WB	Cancer Med; Long noncoding RNA SNHG4 promotes the malignant progression of hepatocellular carcinoma through the miR-211-5p/CREB5 axis; Authors - Jiannan Qiu
human	IF	Sci Rep; Identification of SLC40A1, LCN2, CREB5, and SLC7A11 as ferroptosis-related biomarkers in alopecia areata through machine learning; Authors - Wen Xu
human	WB	Dis Markers; Plasma microRNA-320c as a Potential Biomarker for the Severity of Knee Osteoarthritis and Regulates cAMP Responsive Element Binding Protein 5 (CREB5) in Chondrocytes; Authors - Rongwei Zhou ; KD Validated