Various lysates were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
Various lysates were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
WB analysis using 67617-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HSC-T6 using 67617-1-Ig
HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 67617-1-Ig
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 67617-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
FC experiment of HepG2 using 67617-1-Ig
1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human CREB3L1,OASIS (67617-1-Ig, Clone:2B5B4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human CREB3L1,OASIS (67617-1-Ig, Clone:2B5B4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
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PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Cyclic AMP-responsive element-binding protein 3-like protein 1(CREB3L1) belongs to a family of transcription factors that are synthesized as membrane-bound precursors in the endoplasmic reticulum (ER) and then transported to the Golgi where they are activated through regulated intramembrane proteolysis (RIP). Also it participates in ER-stress response in astrocytes in the central nervous system. It may has a role in gliosis, and involves in activation of transcription through box-B element but not CRE. CREB3L1 acts as a transcription repressor in proliferation of virus-infected cell. The full-length precursor form (approximately 84 kDa) of CREB3L1 and the matured form of CREB3L1 is 58 kDa. (PMID: 20668028)
Various lysates were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
WB analysis using 67617-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HSC-T6 using 67617-1-Ig
HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 67617-1-Ig
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 67617-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
FC (INTRA) Figures
FC experiment of HepG2 using 67617-1-Ig
1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human CREB3L1,OASIS (67617-1-Ig, Clone:2B5B4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
CREB3L1,OASIS Monoclonal antibody
Catalog Number
67617-1-Ig
Citations
-
Dilutions
WB : 1:20000-1:100000 FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
Applications
WB, ELISA, FC (Intra)
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
