CREB3L1,OASIS Polyclonal antibody

• Featured Product
• KD/KO Validated

CREB3L1,OASIS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 11235-2-AP

Synonyms

CREB3L1, OASIS, Processed cyclic AMP-responsive element-binding protein 3-like protein 1, Old astrocyte specifically-induced substance, Cyclic AMP-responsive element-binding protein 3-like protein 1

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Validation Data Gallery

• 

  WB analysis of A549 using 11235-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11235-2-AP (CREB3L1,OASIS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This batch of A549 cells lysate predominantly expressed the maturer form likely due to cell stage.

• 

  WB analysis of COLO 320 using 11235-2-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11235-2-AP (CREB3L1,OASIS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11235-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11235-2-AP (CREB3L1,OASIS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This batch of A549 cells lysate predominantly expressed the precursor form likely due to cell stage.

• 

  IHC staining of human prostate cancer using 11235-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11235-2-AP (CREB3L1,OASIS antibody) at dilution of 1:50 (under 25x lens).

• 

  IF Staining of A549 using 11235-2-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 11235-2-AP (CREB3L1,OASIS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, COLO 320 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Product Information

11235-2-AP targets CREB3L1,OASIS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CREB3L1,OASIS fusion protein Ag1757
• Full Name: cAMP responsive element binding protein 3-like 1
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 55-58 kDa and 84 kDa
• GenBank Accession Number: BC015781
• Gene Symbol: CREB3L1/OASIS
• Gene ID (NCBI): 90993
• RRID: AB_2086042
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclic AMP-responsive element-binding protein 3-like protein 1(CREB3L1) belongs to a family of transcription factors that are synthesized as membrane-bound precursors in the endoplasmic reticulum (ER) and then transported to the Golgi where they are activated through regulated intramembrane proteolysis (RIP). Also it participates in ER-stress response in astrocytes in the central nervous system. It may has a role in gliosis, and involves in activation of transcription through box-B element but not CRE. CREB3L1 acts as a transcription repressor in proliferation of virus-infected cell. The full-length precursor form (approximately 84 kDa) of CREB3L1 and the matured form of CREB3L1 is 58 kDa. (PMID: 20668028)

Publications

Species	Application	Title
	WB	Epigenetics; OASIS/CREB3L1 is epigenetically silenced in human bladder cancer facilitating tumor cell spreading and migration in vitro.; Authors - Michael Rose
rat	WB	Endocrinology; OASIS/CREB3L1 induces expression of genes involved in extracellular matrix production but not classical endoplasmic reticulum stress response genes in pancreatic beta-cells.; Authors - Vellanki Ravi N RN
human	WB,IHC	Breast Cancer Res; Epigenetic silencing of CREB3L1 by DNA methylation is associated with high-grade metastatic breast cancers with poor prognosis and is prevalent in triple negative breast cancers.; Authors - Alison K Ward
human, rat	WB	PLoS One; OASIS/CREB3L1 is induced by endoplasmic reticulum stress in human glioma cell lines and contributes to the unfolded protein response, extracellular matrix production and cell migration.; Authors - Vellanki Ravi N RN ; KD Validated
human	WB,IHC	BMC Cancer; CREB3L1 as a potential biomarker predicting response of triple negative breast cancer to doxorubicin-based chemotherapy.; Authors - Bray Denard
rat	WB	Front Endocrinol (Lausanne); Maternal Protein Restriction Induces Alterations in Hepatic Unfolded Protein Response-Related Molecules in Adult Rat Offspring.; Authors - Xiaomei Liu

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