CRCP Polyclonal antibody

CRCP Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14348-1-AP

Synonyms

CGRP RCP, CGRP receptor component, CGRPRCP, CRCP, HsC17, RCP, RCP9, RNA polymerase III subunit C9



Tested Applications

Positive WB detected inRaji cells, HeLa cells, human testis tissue, MCF-7 cells
Positive IP detected inRaji cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14348-1-AP targets CRCP in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRCP fusion protein Ag5689
Full Name CGRP receptor component
Calculated Molecular Weight 17 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC040107
Gene Symbol CRCP
Gene ID (NCBI) 27297
RRIDAB_10641051
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CRCP antibody 14348-1-APDownload protocol
IHC protocol for CRCP antibody 14348-1-APDownload protocol
IP protocol for CRCP antibody 14348-1-APDownload protocol
FC protocol for CRCP antibody 14348-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,ratIF

J Neuroinflammation

Calcitonin gene-related peptide regulates spinal microglial activation through the histone H3 lysine 27 trimethylation via enhancer of zeste homolog-2 in rats with neuropathic pain.

Authors - Qi An
humanWB

J Transl Med

CircESRP1 enhances metastasis and epithelial-mesenchymal transition in endometrial cancer via the miR-874-3p/CPEB4 axis.

Authors - Rui Shi
ratIF,WB

CNS Neurosci Ther

Calcitonin gene-related peptide induces the histone H3 lysine 9 acetylation in astrocytes associated with neuroinflammation in rats with neuropathic pain.

Authors - Chenyan Sun
mouseIF

Neurogastroenterol Motil

Altered gene expression signatures by calcitonin gene-related peptide promoted mast cell activity in the colon of stress-induced visceral hyperalgesia mice.

Authors - Hanliang Sun
ratIF

Cell Biochem Funct

CNTs-CaP/chitosan-coated AZ91D magnesium alloy extract promoted rat dorsal root ganglia neuron growth via activating ERK signalling pathway.

Authors - Tingjiao Liu
mouseIF

J Immunol Res

ChIP-seq Profiling Identifies Histone Deacetylase 2 Targeting Genes Involved in Immune and Inflammatory Regulation Induced by Calcitonin Gene-Related Peptide in Microglial Cells.

Authors - Xingjing Guo