CRB3 Polyclonal antibody

CRB3 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 12315-1-AP

Synonyms

CRB3, crumbs homolog 3 (Drosophila), Crumbs protein homolog 3

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Validation Data Gallery

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  IHC staining of human pancreas using 12315-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12315-1-AP (CRB3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 12315-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12315-1-AP (CRB3 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human skeletal muscle using 12315-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 12315-1-AP (CRB3 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 12315-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 12315-1-AP (CRB3 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human pancreas tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12315-1-AP targets CRB3 in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CRB3 fusion protein Ag2968
• Full Name: crumbs homolog 3 (Drosophila)
• Calculated Molecular Weight: 123 aa, 13 kDa
• GenBank Accession Number: BC018409
• Gene Symbol: CRB3
• Gene ID (NCBI): 92359
• RRID: AB_2877844
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Crumbs is an apical transmembrane protein crucial for epithelial morphogenesis in Drosophila melanogaster embryos. Three isoforms of mammalian Crumbs have been identified, and two have been characterized. Crumbs1 (CRB1) is expressed primarily in the central nervous system, whereas Crumbs3 (CRB3) is mainly expressed in epithelial tissues and skeletal muscles. CRB3 is an N-glycosylated, small transmembrane protein. CRB3 has a conserved cytoplasmic domain containing the two motives GTY and ERLI found in Crumbs, but exhibits a very short extracellular domain without the EGF- and laminin A-like G repeats present in the other Crumbs. Through its cytoplasmic domain and its interactors (e.g., Pals1 and Par6), CRB3 plays a role in apical membrane morphogenesis and tight junction regulation. (PMID: 14718572; 12527193; 15976445)