RAIDD Polyclonal antibody

• Featured Product
• KD/KO Validated

RAIDD Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 10401-1-AP

Synonyms

CRADD, RAIDD

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Validation Data Gallery

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  WB analysis of K-562 using 10401-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10401-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10401-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 10401-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 10401-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10401-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10401-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 10401-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10401-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10401-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 10401-1-AP

  IP result of anti-RAIDD (IP:10401-1-AP, 3ug; Detection:10401-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

• 

  IHC staining of human colon cancer using 10401-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10401-1-AP (RAIDD antibody) at dilution of 1:100 (under 25x lens).

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Tested Applications

• Positive WB detected in: K-562 cells, HEK-293 cells, HeLa cells, MCF-7 cells, mouse heart tissue, mouse kidney tissue, mouse liver tissue, mouse skeletal muscle tissue, mouse testis tissue, Raji cells
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 6 publications below

Product Information

10401-1-AP targets RAIDD in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAIDD fusion protein Ag0630
• Full Name: CASP2 and RIPK1 domain containing adaptor with death domain
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC017042
• Gene Symbol: CRADD
• Gene ID (NCBI): 8738
• RRID: AB_2085477
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAIDD, also named as CRADD, is an apoptotic adaptor molecule specific for caspase-2 and FASL/TNF receptor-interacting protein RIP. In the presence of RIP and TRADD, CRADD recruits caspase-2 to the TNFR-1 signalling complex. CRADD interacts with BCL10 through its caspase recruitment domain and suppresses interactions between BCL10 and CARMA1. It is a negative regulator of the CARMA1 signalosome and suppressor of Th1-and Th17-mediated inflammatory responses.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Caspase-2 is a condensate-mediated deubiquitinase in protein quality control; Authors - Yingwei Ge
mouse	WB	Dev Cell; E2F-Family Members Engage the PIDDosome to Limit Hepatocyte Ploidy in Liver Development and Regeneration.; Authors - Valentina C Sladky ; KD Validated
mouse	WB	EMBO Rep; PIDDosome-induced p53-dependent ploidy restriction facilitates hepatocarcinogenesis.; Authors - Valentina C Sladky
mouse	WB	J Immunol; Cutting edge: the "death" adaptor CRADD/RAIDD targets BCL10 and suppresses agonist-induced cytokine expression in T lymphocytes.; Authors - Lin Qing Q ; KO Validated
human, mouse	WB	J Biol Chem; The Adaptor CRADD/RAIDD Controls Activation of Endothelial Cells by Proinflammatory Stimuli.; Authors - Huan Qiao
mouse	WB	Cell Metab; PIDDosome-SCAP crosstalk controls high-fructose-diet-dependent transition from simple steatosis to steatohepatitis; Authors - Ju Youn Kim ; KO Validated