CD21 Monoclonal antibody

CD21 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D9A3

Cat no : 66701-1-Ig

Synonyms

C3DR, CD21, Complement C3d receptor, Complement receptor type 2, CR2, EBV receptor, Epstein Barr virus receptor, SLEB9

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Validation Data Gallery

• 

  WB analysis of human spleen using 66701-1-Ig

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66701-1-Ig (CD21 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66701-1-Ig targets CD21 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD21 fusion protein Ag18058
• Full Name: complement component (3d/Epstein Barr virus) receptor 2
• Calculated Molecular Weight: 1092 aa, 119 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC136394
• Gene Symbol: CD21
• Gene ID (NCBI): 1380
• RRID: AB_2882054
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD21, also known as complement receptor type 2 (CR2), complement C3d receptor, and Epstein-Barr virus receptor, is a transmembrane protein that contains a small cytoplasmic domain, a transmembrane region and an extracellular domain consisting of 15 tandem short consensus repeat sequences (PMID: 6230668; 2551147). It is expressed on B cells, follicular dendritic cells, thymocytes and a subset of peripheral T cells (PMID: 26119182). CD21 binds complement fragments C3d, C3dg and iC3b and acts as a receptor for the Epstein-Barr virus (PMID: 7753047). On B cells, together with CD19 and CD81, CD21 forms a complex that functions as a co-receptor to the BCR (PMID: 7542009). It is involved in B cells activation and functions.